体NK细胞转移花素以选择性杀死热囊细胞中的细菌
Ângela C Crespo1, Sachin Mulik2, Farokh Dotiwala2
1Program in Cellular and Molecular Medicine, Boston Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston, MA 02115, USA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA 02138, USA; PhD Programme in Experimental Biology and Biomedicine, Center for Neurosciences and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.
Cell
|August 22, 2020
概括
母体免疫细胞通过纳米管转移抗菌素 (GNLY) 来保护胎盘免受感染. 这种机制可以杀死病原体而不会损害孕产妇与胎儿之间的关系,
科学领域:
- 免疫学
- 生殖生物学
- 微生物学
背景情况:
- 在成功的胎盘形成过程中,母体的果细胞 (dNK) 是至关重要的.
- 确切的dNK细胞保护胎盘免受感染的机制,同时保持胎儿的耐受性仍然不完全理解.
研究的目的:
- 研究dNK细胞在胎盘防御感染中的作用.
- 阐明dNK细胞的抗菌作用机制,重点关注颗粒素 (GNLY) 转移.
主要方法:
- 在人类dNK细胞中分析GNLY表达.
- 通过纳米管向热囊细胞转移的研究.
- 在人类胎盘培养物,热囊细胞系和小鼠模型中评估GNLY对Listeria monocytogenes (Lm) 的疗效.
- 在感染Lm的GNLY转基因和野生类型小鼠中比较妊娠结果.
主要成果:
- 人类dNK细胞表达高水平的GNLY,并通过纳米管将其转移到外流性热囊细胞.
- 转移的GNLY有效地杀死热囊细胞内的Lm,而不会导致热囊细胞死亡.
- GNLY转移显著降低了胎盘和胎儿组织中的Lm负荷,改善了GNLY转基因小鼠的怀孕成功.
- 周围NK细胞也利用GNLY转移去除其他免疫细胞中的细菌.
结论:
- 通过纳米管转移粒素是母体dNK细胞保护胎盘免受细菌感染的关键机制.
- 这种GNLY介导的防御策略保护了母胎屏障的完整性, 确保成功怀孕.
- 这些发现揭示了在母胎界面和其他免疫环境中免疫防御的新途径.
相关概念视频
01:28Cells of the Innate Immune Response
8.3K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
8.3K
01:25Immune Surveillance by NK Cells and Phagocytes
7.6K
Immune surveillance is an integral part of the innate immune system, involving the continuous monitoring of peripheral tissues to detect and respond to pathogens, infected cells, or cancerous cells. This surveillance is conducted primarily by natural killer (NK) cells and phagocytes, which employ distinct but complementary mechanisms to identify and eliminate threats.
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
Natural Killer Cells: The Fast Responders
NK cells are large granular lymphocytes found in the blood and lymphatic system. These...
7.6K
01:27Cytotoxic T Cells-mediated Immune Response
6.3K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
6.3K
01:15Transcytosis of IgG
3.9K
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
3.9K
01:29Immune Response Against Viral Pathogens
1.5K
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
1.5K
01:15Differentiation of Common Myeloid Progenitor Cells
3.7K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.7K