在ELF3中类似子的域作为Arabidopsis的热传感器
Jae-Hoon Jung1,2, Antonio D Barbosa1, Stephanie Hutin3
1Sainsbury Laboratory, University of Cambridge, Cambridge, UK.
Nature
|August 28, 2020
概括
植物通过夜间复合蛋白ELF3感知温度,其多重胺 (polyQ) 重复长度决定了热反应. 这种蛋白质经历了温度诱导的快速相变, 显示出一种新的植物热感应机制.
科学领域:
- 植物生物学
- 分子生物学
- 生物物理
背景情况:
- 温度是影响植物生长和发育的关键因素,气候变化影响植物现象.
- 植物感知温度的分子机制在很大程度上是未知的.
- 晚间复合体,包括ELF3,ELF4和LUX,是植物生理时钟的关键组成部分,并作为温度反应的转录抑制剂.
研究的目的:
- 阐明植物感应温度的分子机制.
- 研究EARLY FLOWERING 3 (ELF3) 蛋白质,特别是其多重胺 (polyQ) 重复和类域 (PrD) 在热反应中的作用.
主要方法:
- 在不同气候的植物中分析ELF3重复长度.
- 生物化学和生物物理测定,包括ELF3 PrD片段的体外相位过渡研究.
- 在体内观察温度诱导的ELF3局部变化 (斑点形成).
主要成果:
- 在ELF3中,polyQ重复的长度与其热响应相关.
- 来自更热气候的植物的ELF3蛋白质的热反应性降低,缺乏可检测的PrD.
- 温度在体内诱导ELF3的快速可逆相变 (斑点形成) 和 PrD在体内液滴形成,这取决于PrD.
结论:
- ELF3的类域对其温度感应能力至关重要.
- 温度诱导的 ELF3 液态相分离是植物热感应的一个新机制.
- 这种机制可以快速调整植物对温度波动的反应.
相关概念视频
Cell Signaling in Plants
6.0K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
6.0K
Riboswitches
9.3K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.3K
Diversity of Archaea IV
310
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
310
Conservation of Protein Domains Over Different Proteins
13.8K
Protein domains are small structurally independent units that are part of a single amino acid chain. Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
13.8K
Diversity of Archaea III
236
Crenarchaeota, a prominent phylum of Archaea, is remarkable for its ability to thrive in extreme environments characterized by high temperatures and acidity. These microorganisms inhabit sulfuric hot springs, volcanic systems, and submarine hydrothermal vents, where temperatures often exceed 100°C. The unique adaptations of Crenarchaeota not only allow survival under such extreme conditions but also provide insights into the mechanisms of life in primordial Earth-like...
236
Hyperthermophilic Bacteria
332
Domain Bacteria includes some unique hyperthermophilic species. They exhibit remarkable adaptations that enable survival in extreme environments.Thermotoga species are rod-shaped, gram-negative, non-sporulating hyperthermophiles that form a sheath-like envelope called a toga. They ferment sugars or starch, producing lactate, acetate, CO₂, and H₂, and can also grow via anaerobic respiration using H₂ and ferric iron. Found in hot springs and hydrothermal vents, over 20% of their...
332


