人类生殖中心在接种流感疫苗后启动记忆和原始B细胞
Jackson S Turner1, Julian Q Zhou2, Julianna Han3
1Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO, USA.
Nature
|September 1, 2020
概括
人类的季节性流感疫苗可以刺激B细胞的反应. 这项研究发现,接种疫苗可以诱导生殖中心反应,产生针对新的表位细胞和扩大保护性抗体的B细胞克隆.
科学领域:
- 免疫学
- 疫苗学
- 病毒学
背景情况:
- 流感病毒对全球公众健康构成重大挑战.
- 季节性流感疫苗主要激活记忆B细胞,导致分泌抗体的等离子体.
- 人类流感疫苗接种引发生殖中心反应的原因尚不清楚.
研究的目的:
- 研究人类接种流感疫苗后生殖中心B细胞反应的动态和特异性.
- 确定流感疫苗是否会引起淋巴结排水中的生殖中心反应.
主要方法:
- 使用超声波引导的细针吸收,进行淋巴结排泄的连续采样.
- 对结合流感疫苗的生殖中心B细胞反应进行分析.
- 评估了B细胞克隆重叠,体突变频率和抗体交叉反应性.
主要成果:
- 疫苗结合的生殖中心B细胞在接种疫苗后一周就被检测到,在一些人中持续长达9周.
- 在生殖中心B细胞克隆和早期循环等离子体之间观察到显著的重叠 (12 - 88%).
- 共享克隆表现出高体质突变并产生广泛交叉反应的抗体,而生殖中心特异性克隆显示出较低的突变率和针对新型表位的菌株特异性抗体.
结论:
- 人类接种流感疫苗可能会引起生殖中心反应.
- 这些反应招募B细胞克隆,能够准以前未被解决的表位.
- 这种过程扩大了疫苗诱导的保护性抗体,可能增强对各种流感菌株的免疫力.
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