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相关概念视频

Transmission Electron Microscopy01:15

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In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
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有效传输电子显微镜细胞-纳米结构界面相互作用的表征

Stella Aslanoglou1,2,3, Yaping Chen1,2,3, Viola Oorschot4,5

  • 1Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, VIC 3052, Australia.

Journal of the American Chemical Society
|September 2, 2020
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概括

研究人员开发了一种使用超薄切片进行传输电子显微镜 (TEM) 的新方法来研究细胞与纳米结构的相互作用. 这种技术增强了对纳米环境的细胞反应的理解.

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科学领域:

  • 生物技术和纳米技术
  • 细胞生物学
  • 材料科学

背景情况:

  • 具有可调节的纳米结构的工程纳米生物接口对于细胞研究至关重要.
  • 基质地形和细胞行为之间的复杂关系需要进一步调查.
  • 目前分析细胞纳米结构相互作用的方法可以引入人工物.

研究的目的:

  • 开发一种新型的实验设计,以制备细胞纳米结构印记的超薄部分 (lamellae).
  • 通过传输电子显微镜 (TEM) 进行细胞和纳米结构之间的界面相互作用的表征.
  • 进一步了解细胞对纳米尺度生物物理和生物化学线索的反应.

主要方法:

  • 提出了一种新的实验设计,用于生成细胞纳米结构印记的超薄段 (lamellae).
  • 用于传输电子显微镜 (TEM) 分析.
  • 专注于粘附细胞和垂直对齐的 (Si) 纳米结构之间的相互作用.

主要成果:

  • 成功生成了细胞纳米结构印记的超薄层.
  • 证明了这种方法对细胞纳米结构接口的TEM特征的有效性.
  • 提供了关于细胞与垂直对齐的Si纳米结构相互作用的详细见解.

结论:

  • 开发的片制备技术可为细胞-纳米结构接口提供高分辨率的TEM成像.
  • 这种方法增强了对细胞如何对纳米物理和化学信号的理解.
  • 预计这些发现将有助于设计改进的细胞操纵技术.