来自天体细胞的血小板衍生的生生长因子驱动了时钟,该时钟计时了培养中的寡类细胞的发展
M C Raff1, L E Lillien, W D Richardson
1MRC Developmental Neurobiology Programme, University College London, UK.
Nature
|June 9, 1988
概括
血小板衍生生长因子 (PDGF) 来自1型天体细胞乘以大鼠视神经中的寡细胞发育. 这种生长因子取代了天体细胞受条件的介质,确保了对原生细胞分化的适当时机.
科学领域:
- 发育神经生物学的发展神经生物学.
- 细胞分化的时间表
- 质细胞的发展.
背景情况:
- 多细胞动物表现出可预测的细胞分化时间表,但基本的时间机制在很大程度上是未知的.
- 寡 dendrocytes和类型-2 星球细胞源于正在发育的老鼠视神经中的双电位质 (O-2A) 先生的细胞.
- 在体外,O-2A原始体在没有特定的条件因素的情况下过早分化.
研究的目的:
- 调查控制小干细胞与O-2A原生细胞分化时间的机制.
- 在体外识别恢复正常增殖和分化时间表的因素.
- 确定血小板衍生生长因子 (PDGF) 在质细胞发育时间中的作用.
主要方法:
- 在无血清介质中培养O-2A原始细胞,而不是在1型天体细胞条件下培养的介质中培养O-2A.
- 测试纯化血小板衍生生长因子 (PDGF) 替代天体细胞受条件介质的能力.
- 使用抗PDGF抗体来阻止条件介质的作用.
- 在发育中的视神经中检测PDGF的存在.
主要成果:
- 无血清培养导致O-2A原体过早分化为寡细胞.
- 类型-1 星细胞受条件介质恢复了正常的增殖,并在几周内延长了寡细胞的发展.
- 纯化PDGF模仿了天体细胞受条件介质的影响,而抗PDGF抗体则中和了这种效应.
- 在成长中的老鼠视神经中检测到PDGF.
结论:
- 从1型天体细胞衍生的PDGF充当了对寡头细胞发育的关键时间信号.
- 早期分化的1型天体细胞释放的PDGF驱动了寡基细胞原生细胞的发育时钟.
- 这项研究阐明了一种关键的分子机制,该机制调节了活体内质细胞分化的精确时间.
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