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相关概念视频

Rab Proteins01:14

Rab Proteins

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Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
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Adrenergic Receptors: β Subtype01:26

Adrenergic Receptors: β Subtype

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β-adrenoceptors have varied sensitivities towards adrenaline, noradrenaline, and isoprenaline. The order of agonist potency is as follows:
Isoprenaline > Adrenaline > Noradrenaline
Neurotransmitter binding to these receptors causes activation of adenylyl cyclase resulting in increased concentrations of cAMP and modulation of calcium ion channels within the cell. They are further classified into β1, β2, and β3 subtypes.
β1-adrenoceptors: β1-adrenoceptors...
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Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

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Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
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Aquaporins01:25

Aquaporins

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Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
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Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

6.7K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
6.7K
Rab Cascades01:25

Rab Cascades

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Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
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Monitoring GPCR-β-arrestin1/2 Interactions in Real Time Living Systems to Accelerate Drug Discovery
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快照:β-逮捕函数

Seungkirl Ahn1, Sudha K Shenoy2, Louis M Luttrell3

  • 1Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

Cell
|September 5, 2020
PubMed
概括

阿雷斯是关键的适应蛋白,参与由G蛋白结合受体启动的信号通路. 这些蛋白质在阻断受体-G蛋白相互作用之外发挥动态作用,影响各种细胞功能.

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Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
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Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
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Parallel Interrogation of β-Arrestin2 Recruitment for Ligand Screening on a GPCR-Wide Scale using PRESTO-Tango Assay
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科学领域:

  • 细胞生物学
  • 分子信号
  • 生物化学

背景情况:

  • 阿雷斯是细胞中无处不在的适应蛋白.
  • 已知它们与7跨膜G蛋白结合受体 (GPCR) 相互作用.
  • 最初通过阻断G蛋白合来降低GPCR的敏感性.

研究的目的:

  • 突出了人们对阿斯特林功能的不断变化的理解.
  • 为了强调阿雷斯的动态蛋白相互作用.
  • 探索阿雷斯在GPCR信号传递之外的细胞作用.

主要方法:

  • 关于逮捕的文献综述
  • 蛋白质相互作用研究的分析.
  • 对信号通路组件的研究.

主要成果:

  • 阿雷斯参与多种跨膜信号级联.
  • 阿雷斯具有广泛的动态蛋白相互作用.
  • 在GPCR脱敏之外,阿雷斯调节多种细胞功能.

结论:

  • 阿雷斯是多功能适应蛋白质,在细胞信号传递中具有多方面的作用.
  • 阿雷斯的功能范围正在不断扩大.
  • 对于解读复杂的细胞过程至关重要.