在人体细胞中脱离过程中激活cdc2蛋白激酶:细胞周期依赖的酸化和子单元重组
概括
这项研究研究了HeLa细胞p34,一种参与细胞循环调节的蛋白激酶. 它的活性和与其他蛋白质的关联在整个细胞周期中发生变化,在线粒分裂期间达到峰值,以调节G2/M过渡.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- p34是一种酵母cdc2+/cdc28蛋白激酶的同源.
- p34与p13形成一个复合体,这是S. pombe suc1+的同类物.
- p34与新发现的蛋白质p62相关,该蛋白质在体外被酸化.
研究的目的:
- 研究HeLa细胞p34在细胞循环调节中的作用.
- 描述p34.4的酸化状态和复杂形成.
- 为了确定p34/p62复合体的细胞周期依赖活性.
主要方法:
- 研究的p34酸化状态.
- 分析了p34与p13和p62.6的关联.
- 在试验室中评估p34复合物的蛋白激酶活性.
- 将这些特性与细胞周期阶段 (G1,G2,M) 相关联.
主要成果:
- p34在多个部位被酸化,并与p13和p62形成复合体.
- 对于p34的酸化状态,与p62的关联,以及其激酶活性都是细胞周期调节的.
- 在G1中,p34是非酸化的,不与p62结合,并且不活跃.
- 在G2和M阶段,p34高度酸化,与p62相关,并表现出最大的激酶活性.
- 在线粒转化阶段,p34/p62复合体在线粒转化阶段表现出最大的活动.
结论:
- p34,与p62的复合体中,作为细胞循环调节的蛋白激酶.
- p34/p62复合体可能在调节HeLa细胞中的G2/M过渡中发挥关键作用.
更多相关视频
08:33Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
Published on: December 5, 2017
12:26Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
相关概念视频
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...