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相关概念视频

Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
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Translational Regulation01:29

Translational Regulation

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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
422
Experimental RNAi02:15

Experimental RNAi

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Cis-regulatory Sequences02:02

Cis-regulatory Sequences

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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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相关实验视频

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Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
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Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen

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C. elegans解释细菌非编码RNA以学习致病性回避

Rachel Kaletsky1,2, Rebecca S Moore1, Geoffrey D Vrla1

  • 1Department of Molecular Biology, Princeton University, Princeton, NJ, USA.

Nature
|September 10, 2020
PubMed
概括
此摘要是机器生成的。

一次接触致病细菌

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科学领域:

  • 微生物学
  • 遗传学
  • 神经科学

背景情况:

  • 它必须区分食物和病原体.
  • 细菌暴露会影响虫的行为.

研究的目的:

  • 研究小RNAs在病原体回避学习中的作用.
  • 确定避免行为的跨代遗传机制.

主要方法:

  • 对C. elegans暴露于致病性Pseudomonas aeruginosa (PA14) 小RNA.
  • 评估治疗的虫和后代的避开行为.
  • 研究RNA干扰 (RNAi),PIWI相互作用RNA (piRNA) 途径,生殖系和ASI神经元的参与.
  • 识别特定的细菌非编码RNA及其C. elegans点.

主要成果:

  • 一次接触PA14小RNA诱导了C. elegans的病原体回避.
  • 这种学会避免是继承了四代人的.
  • RNAi和piRNA通路,生殖系和ASI神经元对于诱导回避及其遗传都至关重要.
  • 一个特定的Pseudomonas aeruginosa非编码RNA (P11) 是必要的,并且足以避免学习.
  • 鉴定出C. elegans基因maco-1是这种回避行为所需的目标.

结论:

  • 在C. elegans中,小RNA调解了学会的病原体回避.
  • 这种学习的行为及其潜在的分子机制是跨代遗传的.
  • 一种非编码RNA依赖的机制使虫能够感知和传递有关微生物威胁的信息.