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氨基酸衍生物的核糖体延长

Takayuki Katoh¹, Hiroaki Suga¹

¹Department of Chemistry, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Journal of the American Chemical Society

|September 18, 2020

概括

研究人员成功地将芳香氨基酸结合到中, 这一突破使得新型和具有独特特性的大环的合成成为可能.

科学领域:

合成生物学
蛋白质工程
生物化学

背景情况:

2-氨基酸和异环氨基酸在结构上与β氨基酸相似,但具有不同的化学性质.
它们的氨基基群的核友性较差,阻碍了核酶体的纳入链.
通过核糖体合成将这些芳香氨基酸纳入新生链的先前尝试没有成功.

研究的目的:

开发一种将二氨基酸和异环氨基酸纳入的方法.
证明合成含有这些非正规氨基酸的和宏环的可行性.

主要方法:

使用了与设计器tRNA (tRNAPro1E2) 复合的翻译系统.
设计了tRNAPro1E2以调用延长因子EF-Tu和EF-P.
用二氨基酸和异环氨基酸的衍生物充电设计器tRNA.

主要成果:

成功地将2-氨基酸和异环氨基酸纳入新生的链.
获得含有这些芳香氨基酸的的成功表达.
证明了包含这些奇特氨基酸的宏环的合成.

结论:

开发的复制翻译系统可使芳香氨基酸在核糖体内结合起来.
这种方法为产生具有新结构和功能的和宏环开辟了新的途径.
扩大可用于合成的氨基酸范围.

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