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人类视网膜的细胞类型及其单细胞分辨率
Cameron S Cowan1, Magdalena Renner2, Martina De Gennaro3
1Institute of Molecular and Clinical Ophthalmology Basel, 4031 Basel, Switzerland; Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland.
Cell
|September 18, 2020
概括
研究人员开发出具有光敏度的人类视网膜器官,模仿成人视网膜细胞类型和功能. 这些器官为研究视网膜疾病和确定新的治疗点提供了有价值的模型.
科学领域:
- 生物技术
- 神经科学
- 眼科 眼科
背景情况:
- 人类器官对于理解器官发育和疾病至关重要.
- 开发功能性的视网膜器官对于视觉研究至关重要.
研究的目的:
- 制造出对光敏感的人类视网膜有机体,
- 将视网膜器官的细胞和分子发育与成年人视网膜进行比较.
- 为疾病研究确定细胞类型特定的基因表达模式.
主要方法:
- 具有层次结构和功能突触的人类视网膜器官的发展.
- 在七个发育时间点从有机体中测序285,441个单细胞RNA.
- 机体和成年人视网膜组织 (外周,膜,色素上皮,胸膜) 的组织化学分析.
主要成果:
- 在体外成熟的器官细胞类型与人体视网膜发育的速度相当.
- 有机细胞的转录组汇聚到成年外围视网膜细胞类型.
- 细胞类型特定的与疾病相关的基因表达在有机体中保持,这涉及到像黄斑变性等疾病中的新型细胞类型.
结论:
- 已开发的视网膜器官可以作为研究人类视网膜发育和功能的强大模型.
- 这种资源为研究疾病机制和视网膜疾病的潜在治疗干预提供了细胞点.
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