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相关概念视频

Conserved Binding Sites01:49

Conserved Binding Sites

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Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
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Conserved Binding Sites01:49

Conserved Binding Sites

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Lipids as Anchors01:32

Lipids as Anchors

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In the plasma membrane, the lipids forming the bilayer can also act as an anchor to tether proteins to the membrane. The three main types of lipid anchors found in eukaryotes are – prenyl groups, fatty acyl groups, and glycosylphosphatidylinositol or GPI groups. Prenyl and fatty acyl groups act as anchors on the cytosolic surface of the membrane, whereas GPI anchors proteins on the extracellular side.
The carboxy-terminal of most of the prenylated proteins, such as Ras proteins, contains...
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Conjugated Proteins02:50

Conjugated Proteins

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Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
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Ligand Binding Sites

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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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Protein Folding01:22

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Overview
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在SARS-CoV-2尖端蛋白的锁定结构中的自由脂肪酸结合口袋

Christine Toelzer1,2, Kapil Gupta1,2, Sathish K N Yadav1,2

  • 1School of Biochemistry, University of Bristol, 1 Tankard's Close, Bristol BS8 1TD, UK.

Science (New York, N.Y.)
|September 22, 2020
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概括

酸 (LA) 与SARS-CoV-2尖端蛋白结合,稳定其结构并减少ACE2的相互作用. 这一发现可能会导致针对COVID-19的新治疗策略.

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科学领域:

  • 结构生物学
  • 病毒学
  • 生物化学

背景情况:

  • 冠状病毒疾病2019 (COVID-19) 是由SARS-CoV-2引起的全球性健康危机.
  • 了解SARS-CoV-2的机制对于开发有效的治疗方法至关重要.
  • 尖峰 (S) 糖蛋白驱动病毒感染性和病理.

研究的目的:

  • 阐明SARS-CoV-2感染性的结构基础.
  • 研究自由脂肪酸在S糖蛋白功能的作用.
  • 为了确定COVID-19的潜在治疗点.

主要方法:

  • 在2.85安格斯特罗姆分辨率的冷电子显微镜.
  • 对SARS-CoV-2 S糖蛋白的结构分析.
  • 在体外测试以评估ACE2相互作用和病毒复制.
  • 在人体细胞中补充酸.

主要成果:

  • 在SARS-CoV-2 S糖蛋白中,有三个结合酸 (LA) 的口袋.
  • LA结合使S糖蛋白稳定在一个"锁定"的形状.
  • 这种形状在体外减少了与血管素转化酶2 (ACE2) 的相互作用.
  • LA与雷梅西维尔协同作用,抑制SARS-CoV-2在人体细胞中的复制.
  • 在SARS-CoV和MERS-CoV中建议使用类似的LA结合口袋.

结论:

  • 酸与SARS-CoV-2尖端蛋白直接相互作用.
  • LA结合是一种影响病毒进入和稳定的新机制.
  • 针对LA结合提供了针对COVID-19和相关冠状病毒的潜在治疗策略.