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通过结构指导的高分辨率血清学绘制SARS-CoV-2尖端受体结合域上的中和和免疫主导部位

Luca Piccoli¹, Young-Jun Park², M Alejandra Tortorici³

¹Humabs BioMed SA, Vir Biotechnology, 6500 Bellinzona, Switzerland.

|September 29, 2020

概括

针对SARS-CoV-2受体结合域 (RBD) 的中和抗体 (nAbs) 是免疫的关键. 这项研究绘制了RBD表征,指导了未来的COVID-19疫苗和治疗开发.

科学领域:

免疫学
病毒学
疫苗学

背景情况:

了解SARS-CoV-2中和抗体 (nAbs) 对免疫保护和疫苗开发至关重要.
尖峰 (S) 和核蛋白是SARS-CoV-2感染后抗体 (Ab) 反应的关键目标.

研究的目的:

分析SARS-CoV-2感染引起的nAbs的特异性和动力学.
识别SARS-CoV-2蛋白质组中的免疫主导点并绘制它们的抗原位点.
以指导有效的COVID-19疫苗和治疗方法的设计.

主要方法:

在647名SARS-CoV-2感染者中分析抗体 (Ab) 反应.
中和抗体 (nAb) 标位和度的量化.
RBD抗原图的结构定义和表位特异性Abs的血清定量.

主要成果:

在感染者中,Ab数量和nAb数量都与临床评分相关.
受体结合域 (RBD) 是免疫主导的,负责90%的中和活性.
虽然整体RBD特异性IgG标位下降,但nAb标位和性在一些人中增加,表明亲和力成熟.
确定了两个主要的受体结合基因抗原位点.

结论:

该RBD是对抗SARS-CoV-2抗体中和的主要目标.
抗体反应表现出复杂的动力学,包括潜在的亲和力成熟.
绘制RBD抗原点为开发针对性的COVID-19疫苗和治疗方法提供了关键的见解.

关键词:

美国冠状病毒冠状病毒效应器功能豁免权中和抗体

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