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相关概念视频

Yeast Signaling01:28

Yeast Signaling

Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...

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相关实验视频

Updated: Jul 7, 2026

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

一个酵母操作员重叠一个上游激活站点.

J W Kronstad, J A Holly, V L MacKay

    Cell
    |July 31, 1987
    PubMed
    概括

    酵母A细胞中的BAR1基因产物使α因子失活. 它的转录由MATα2蛋白和α因子调节,影响酵母交配和基因调节.

    科学领域:

    • 分子生物学分子生物学
    • 酵母遗传学 酵母遗传学
    • 基因规则 基因规则

    背景情况:

    • 在Saccharomyces cerevisiae中的BAR1基因仅在a细胞中表达,并编码一种蛋白质,该蛋白质使α-因子失活,α-细胞产生的交配费洛蒙.
    • 酵母交配中的关键调节者MATα2蛋白抑制α细胞特异基因,包括BAR1,在α和α/α双倍细胞中.
    • 在a细胞中,BAR1转录在暴露于α因子时被诱导,这表明一个复杂的调节网络.

    研究的目的:

    • 研究控制Saccharomyces cerevisiae中BAR1基因表达的调控机制.
    • 确定负责MATα2介导抑制和alpha因子诱导的BAR1转录激活的特定DNA序列.
    • 了解这些发现对酵母中基因转录的负控制的影响.

    主要方法:

    • 对BAR1基因5'非编码区域的删除分析.
    • 对基因转录的分析,以应对特定的交配因素和调节性蛋白质.

    主要成果:

    • 删除分析显示,BAR1的主要上游激活部位 (UAS) 与MATα2抑制所必需的31bp操作器序列重叠.
    • 鉴定出TGAAACA序列是介导BAR1.1的α因子刺激转录的.
    • 这些发现突出了监管重叠对于控制酵母中的基因表达至关重要.

    更多相关视频

    Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
    08:48

    Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

    Published on: January 26, 2017

    Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
    07:18

    Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

    Published on: May 15, 2018

    相关实验视频

    Last Updated: Jul 7, 2026

    Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
    09:22

    Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

    Published on: November 26, 2013

    Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast
    08:48

    Targeted in Situ Mutagenesis of Histone Genes in Budding Yeast

    Published on: January 26, 2017

    Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
    07:18

    Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast

    Published on: May 15, 2018

    结论:

    • 这项研究阐明了BAR1基因表达的复杂调节,涉及MATα2的抑制和α-因子的激活.
    • 抑制和激活的重叠监管元素对理解酵母中负转录控制有重大影响.
    • 已识别的TGAAACA序列是a细胞中激素诱导的基因表达的关键媒介.