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低于1 Å的样本移动的冷EM

Katerina Naydenova1, Peipei Jia1,2, Christopher J Russo3

  • 1MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK.

Science (New York, N.Y.)
|October 9, 2020
PubMed
概括

在低温电子显微镜 (cryo-EM) 中的粒子运动是由冰的变形引起的. 在辐射损伤发生之前, 一个新的样本支设计可以最大限度地减少这种运动, 实现更高质量的冷电磁成像和分析.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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