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乳酸激发ER-线粒体Mg2+动力学,以整合细胞代谢

Cassidy C Daw¹, Karthik Ramachandran¹, Benjamin T Enslow¹

¹Department of Medicine, Center for Precision Medicine, University of Texas Health San Antonio, San Antonio, TX 78229, USA; Department of Medicine/Cardiology/Diabetes/Nephrology Divisions, University of Texas Health San Antonio, San Antonio, TX 78229, USA.

|October 9, 2020

概括

乳酸酸激发细胞内 (Mg2+) 从细胞内网膜迅速释放,增强线粒体Mg2+吸收. 这个过程对温度敏感,可以缓解多器官衰竭.

科学领域:

细胞生物学细胞生物学
生物化学生物化学
生理学生理学生理学

背景情况:

(Mg2+) 对于细胞功能至关重要,作为酶和核酸的辅因子.
了解细胞内Mg2+ (iMg2+) 动态的调节是解读细胞信号通路的关键.

研究的目的:

通过一个全面的屏幕来识别iMg2+动态的调节者.
研究乳酸在调节细胞间Mg2+运输中的作用.

主要方法:

使用全面的选方法来识别iMg2+监管者.
研究了从内质网膜 (ER) 储存中的Mg2+释放和线粒体Mg2+ (mMg2+) 吸收.
评估了参与乳酸介导Mg2+动态的温度敏感性和信号通路.
研究了Mrs2蛋白在乳酸诱导的mMg2+吸收中的作用.

主要成果:

乳酸盐被确定为从ER储存中快速释放Mg2+的激活剂.
这种ER-to-mitochondrialMg2+流是温度敏感的,并通过细胞内信号进行介导.
L-乳酸盐可以选择性地刺激ER-线粒体Mg2+动态.
Mrs2蛋白促进乳酸介导的mMg2+进入,而特定的突变会破坏这一过程.
抑制mMg2+的激增减轻了炎症诱导的多器官衰竭.

结论:

乳酸盐通过促进ER释放和随后的线粒体吸收来调动iMg2+ .
这种Mg2+运输与代谢反回路和线粒体生物能学有关.
针对乳酸介导的Mg2+动态提供了潜在的炎症状况治疗策略.

关键词:

这位女士是 Mrs2 Mrs2 和是最重要的.癌症癌症癌症癌症癌症道道道道道细胞内膜网膜的内oplasmic网膜.这是一种炎症炎症炎症炎症.乳酸乳酸是一种乳酸.的含量是多少? 的含量是多少?代谢代谢代谢代谢线粒体中的线粒体.

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