相关实验视频

Updated: Dec 6, 2025

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

A Pipeline to Investigate the Structures and Signaling Pathways of Sphingosine 1-Phosphate Receptors

Published on: June 8, 2022

在活细胞中检测小分子化探针

Andrew K Rudd¹, Neel Mittal¹, Esther W Lim²

¹Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093, United States.

Journal of the American Chemical Society

|October 12, 2020

概括

研究人员开发了一种光探测器, 这种新工具有助于研究.

科学领域:

生物化学
细胞生物学
化学生物学

背景情况:

甲素是一种关键的单链甲脂,参与细胞结构和信号传递.
在癌症,糖尿病和阿尔茨海默病等疾病中,
现有的方法缺乏选择性,活细胞检测疏水性脂的工具.

研究的目的:

开发一种新型的光启动探测器,
验证探测器对内源性菌素的特异性和敏感性.
将探针应用于涉及氨酸积累的疾病模型.

主要方法:

一个小分子光启动探针的开发.
测试探针对不同度的反应.
用探针检测患者衍生细胞中的斯芬戈辛.

主要成果:

开发的探针显示了对sphingosine的剂量依赖的光反应.
探测器成功地标记了活细胞中的内源性蛋白池.
在使用探针的尼曼·皮克型C1患者的细胞中检测到斯芬戈辛的积累.

结论:

一个新的,与活细胞相容的光探针已经被创造出来.
这种探测器提供了一种简单且易于使用的探测方法.
这种工具将促进对细胞过程和疾病中的斯芬戈辛作用的研究.

更多相关视频

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes

Published on: October 15, 2016

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

相关概念视频

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...