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细菌颗粒含有循环多酸盐:P固态NMR的证据

Venkata S Mandala¹, Daniel M Loh², Scott M Shepard¹

¹Department of Chemistry, Massachusetts Institute of Technology, 170 Albany Street, Cambridge, Massachusetts 02139, United States.

Journal of the American Chemical Society

|October 19, 2020

概括

这项研究为整个细菌细胞中自然存在的循环酸盐提供了第一个明确的证据. 这些发现表明甲酸盐起着重要的生物学作用,挑战了关于多酸盐结构的先前假设.

科学领域:

生物化学
分子生物学
微生物学

背景情况:

多酸盐 (PolyPs) 是有机体中必不可少的聚合物,传统上被视为线性链.
最近的研究表明循环形酸盐可能与酶相互作用,质疑它们的自然存在.
之前关于甲酸盐的报道经常被视为提取方法的文物.

研究的目的:

使用非破坏性方法研究整个细菌细胞中的多酸盐的化学结构.
为了确定循环形酸盐是否在细菌中自然存在.
探索甲酸盐的潜在生物学意义和细胞局部化.

主要方法:

在整个细胞和不溶解部分上采用非破坏性固态核磁共振 (NMR) 光谱.
分析同位素和异位素P化学转移以确定酸盐结构.
已知结构的合成甲酸盐与细胞光谱进行比较以进行识别.

主要成果:

核磁共振 (NMR) 数据显示,在*X. autotrophicus*的水化全细胞中,线性酸盐和循环酸盐并存.
在特定的生长和样本条件下检测到大量循环酸盐,平均尺寸为3-8酸盐单位.
发现甲基酸盐在不溶性,电子密度较高的颗粒中被丰富,外源性六甲基酸盐被代谢为三甲基酸盐.

结论:

这项研究提供了第一个关于整个细菌细胞中生物相关甲酸盐存在的明确证据.
这些发现表明甲酸盐度可以在新鲜细胞中显著,并表明潜在的生物作用.
开辟了研究多种生物中的甲酸盐功能的新途径.

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