靠近结肠的O-糖化粘液封装并调节微生物群
Kirk Bergstrom1,2, Xindi Shan3, David Casero4
1Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA. lijun-xia@omrf.org kirk.bergstrom@ubc.ca.
Science (New York, N.Y.)
|October 23, 2020
概括
结肠粘液组织成两层,微生物群通过近邻结肠杯细胞影响其自身的封装. 这一过程调节了肠道微生物群结构和宿主-粘液相互作用.
科学领域:
- 胃肠病学和微生物学
- 粘膜免疫学
- 主体与微生物群的相互作用
背景情况:
- 结肠粘液层将肠道微生物与宿主组织分开, 但其组织原理和功能调节仍然不完全理解.
- 粘液糖蛋白,特别是Muc2,是粘液屏障的关键组成部分,但它们在组织粘液结构和与微生物群相互作用中的具体作用尚未完全阐明.
研究的目的:
- 研究结肠粘液的结构组织及其对宿主微生物共生的功能影响.
- 确定微生物群如何影响粘液的产生和组成.
- 阐明Muc2中O-甘氨酸在调节微生物群和宿主反应中的作用.
主要方法:
- 大肠粘液产生的小鼠模型.
- 在Muc2中分析O-糖化模式.
- 微生物组合和功能分析.
- 对结肠粘膜进行转录分析.
主要成果:
- 结肠粘液包括两种不同的O-糖化Muc2形式:主要的近位形式封装微生物群和附着的较小的远位形式.
- 肠道微生物群通过近邻结肠杯细胞积极诱导Muc2的产生,从而指导其自身的封装.
- 靠近结肠的Muc2 O- glycans显著调节微生物群的结构和功能,并影响结肠粘膜中的基因表达.
结论:
- 临近结肠粘液的产生是宿主微生物共生中的关键调节机制.
- 结肠粘液的独特组织受微生物群的影响, 在维持肠道平衡方面起着至关重要的作用.
- 针对Muc2 O- 糖化是一种调节肠道微生物群和宿主微生物相互作用的潜在策略.
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