snRNA-seq揭示了调节热生成的脂肪细胞子群
Wenfei Sun1, Hua Dong2, Miroslav Balaz2
1Institute of Food, Nutrition and Health, ETH Zurich, Schwerzenbach, Switzerland. wenfei-sun@ethz.ch.
Nature
|October 29, 2020
概括
研究人员发现了一种罕见的脂肪细胞亚群,通过乙信号调节热生成能力. 这一发现可能解释人类与小鼠脂肪组织的差异,并为恢复热生成提供了一个目标.
科学领域:
- 代谢生物学
- 脂肪组织研究
- 细胞信号传输
背景情况:
- 脂肪组织分为白色,棕色或色 (英),对全身代谢至关重要.
- 肥胖患者的脂肪组织功能障碍会导致代谢并发症.
- 脂肪组织作为一个信号中心,通过对和内分泌信号调节新陈代谢.
研究的目的:
- 在小鼠和人类中使用单核RNA测序 (snRNA-seq) 来描述脂肪细胞异质性.
- 识别新型脂肪细胞亚群及其功能作用.
- 研究脂肪组织中热生成能力的调节.
主要方法:
- 在小鼠和人类脂肪组织上进行了单核RNA测序 (snRNA-seq).
- 分析的重点是识别和量化不同的脂肪细胞亚群.
- 使用功能性测试来评估已识别的亚群体在发热过程中的作用.
主要成果:
- 在小鼠中发现了一种罕见的脂肪细胞亚群,在较高的温度下其数量增加.
- 这种亚群通过酸中介调节热生成能力来调节邻近的脂肪细胞活动.
- 与小鼠相比,人类脂肪组织的这一亚群数量更高.
结论:
- 已识别的脂肪细胞亚群在调节热生成活性方面发挥着关键作用.
- 与小鼠相比,这种亚种群的丰度差异可能解释了人类脂肪组织中较低的热生成活性.
- 向这种乙介导途径为恢复热生成活性提供了潜在的策略.
更多相关视频
11:11Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting
Published on: March 17, 2023
2.6K
06:08Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
Published on: May 19, 2023
2.7K
相关概念视频
03:21RNA-seq
11.3K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
11.3K
01:29Translational Regulation
403
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
403