相关实验视频

Updated: Dec 3, 2025

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

单细胞转录学揭示了肝细胞和非肝细胞系的早期出现

Jeremy Lotto¹, Sibyl Drissler¹, Rebecca Cullum²

¹Terry Fox Laboratory, BC Cancer, Vancouver, BC V5Z 1L3, Canada; Cell and Developmental Biology Program, University of British Columbia, Vancouver, BC V6T 1Z3, Canada.

|October 30, 2020

概括

这项研究利用单细胞转录学绘制了早期肝脏发育的地图,揭示了器官生成过程中不同的细胞类型和相互作用. 它提供了从胚胎日7.5到10.5建立肝脏血统的详细地图.

科学领域:

发育生物学
基因组学
细胞生物学

背景情况:

早期肝脏器官生成是复杂的,限制了细胞出现的详细分析.
了解肝脏发育对于再生医学和疾病研究至关重要.

研究的目的:

创建从胚胎第7.5天到第10.5天的早期肝脏发育的综合单细胞地图.
描述各种肝细胞系的出现和转录特征.

主要方法:

分析了45,334个单细胞转录组.
在肝脏器官生成过程中对细胞群的时空分析.

主要成果:

在胚胎8.75日之前,血管和鼻腔内皮的详细分离.
显著的中细胞和肝星细胞类型的特征.
肝细胞特异性,迁移和集体细胞迁移的识别,包括肝细胞类型.
在原始鼻状组织过程中对细胞相互作用的洞察.

结论:

这项研究提供了从内皮和中皮建立肝脏血统的高分辨率图谱.
它阐明了早期肝脏器官生成和原始鼻状形成期间的关键细胞事件和相互作用.

关键词:

皮肤内皮肝细胞细胞肝脏半体机体发生 scRNA-seq 在一个细胞鼻状内皮星状细胞

更多相关视频

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics

Published on: December 9, 2022

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Hepatic Progenitor Specification from Pluripotent Stem Cells using a Defined Differentiation System

Published on: May 10, 2020

相关概念视频

Cell Specific Gene Expression

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...

Lineage Commitment

Lineage Commitment

Commitment is the process whereby stem cells: