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相关概念视频

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RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
Transcription can be divided into three main stages, each involving distinct DNA sequences to guide the polymerase. These are:
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The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
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While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
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相关实验视频

Updated: Nov 30, 2025

Polysome Fractionation and Analysis of Mammalian Translatomes on a Genome-wide Scale
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哺乳动物中的转录组和转录组共同进化

Zhong-Yi Wang1, Evgeny Leushkin2, Angélica Liechti3

  • 1Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance, Heidelberg, Germany.

Nature
|November 12, 2020
PubMed
概括

这项研究揭示了基因表达在翻译组 (蛋白质合成) 层面比转录组 (RNA) 层面发展得更缓慢,特别是对于必需的基因. 转化调节缓冲变化, 保持物种间的重要功能.

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科学领域:

  • 进化生物学
  • 分子生物学
  • 基因组学

背景情况:

  • 基因表达程序塑造了表型,但它们在RNA之外的进化动态尚不清楚.
  • 了解不同分子层的共同进化对于进化见解至关重要.

研究的目的:

  • 在多种哺乳动物物种和器官中研究转化体和转录体的共同进化.
  • 确定基因表达层之间的缓冲程度及其对进化速率的影响.

主要方法:

  • 来自五种哺乳动物和一只鸟类的大脑,肝脏和丸的核糖体和RNA测序数据.
  • 对转录组和转录组水平的基因表达差异进行比较分析.
  • 质谱蛋白质组学数据的整合.

主要成果:

  • 转化调节是广泛存在的,特别是在丸精子生成细胞中.
  • 由于缓冲,保存重要的基因,转录组分离比转录组分离低20%左右.
  • 转化上调补偿了性别染色体的剂量变化和介质性失活.
  • 测试结果显示基因在翻译层面的进化速度最快,

结论:

  • 基因表达层表现出共同进化的模式,缓冲保护了必需的基因.
  • 转化调节在缓冲进化变化和补偿特定遗传挑战方面发挥着关键作用.
  • 转录组和转录组进化之间的相互作用反映在蛋白质组层.