一个转录抑制器网络调节了auxin反应
Jekaterina Truskina1,2, Jingyi Han2, Elina Chrysanthou2
1Laboratoire Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon 1, CNRS, INRAE, Lyon, France.
Nature
|November 19, 2020
概括
植物激素辅酶通过辅酶反应因子 (ARF) 转录因子调节生长. 这项研究表明,抑制剂控制了Arabidopsis中的A类ARF基因表达,在发育过程中调节了auxin信号.
科学领域:
- 植物生物学
- 分子遗传学
- 发育生物学
背景情况:
- 素是调节生长和发育的关键植物激素.
- 素反应因子 (ARF) 转录因子介导素信号传递.
- 类AARF激活了辅酶反应基因,但它们的表达调节是未知的.
研究的目的:
- 调查Arabidopsis thaliana中A类ARF基因表达的调节.
- 确定控制A类ARF基因的转录调节剂.
- 了解这些调节器如何调节辅酶信号.
主要方法:
- 染色体修饰和可访问性测试
- 酵母的一种混合选以确定转录调节剂.
- 在植物中进行过渡转化和突变表达分析.
主要成果:
- 类A ARF位点是构成性地开放的转录.
- 对每个A类ARF基因确定了特定的转录调节剂.
- 大多数已识别的调节剂作为A类ARF基因转录的抑制剂.
结论:
- 一个转录抑制器网络调节A类ARF表达.
- 开放的染色体允许精确控制ARF水平.
- 这种调节机制在植物发育过程中微调了辅酶信号输出.
相关概念视频
RNA Polymerase II Accessory Proteins
10.3K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
10.3K
Co-activators and Co-repressors
8.2K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.2K
Co-activators and Co-repressors
2.7K
2.7K
Prokaryotic Transcriptional Activators and Repressors
24.5K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
24.5K
Prokaryotic Transcriptional Activators and Repressors
9.9K
9.9K
Eukaryotic Transcription Inhibitors
10.6K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
10.6K


