Abl 和 Canoe/Afadin 在三细胞连接处介导机械传导
Huapeng H Yu1, Jennifer A Zallen2
1Howard Hughes Medical Institute and Developmental Biology Program, Sloan Kettering Institute, New York, NY, USA.
Science (New York, N.Y.)
|November 27, 2020
概括
科学家们发现了一种新的途径,在果胚胎中,Abl 氨酸激酶和Canoe/Afadin 稳定细胞粘附,揭示了细胞在组织发育过程中如何对机械力量做出反应.
科学领域:
- 细胞生物学
- 发育生物学
- 生物物理
背景情况:
- 皮质组织在机械作用下动态地重组细胞.
- 在细胞之间传递力量的关键是附着结.
- 细胞力感应和反应的体内机制尚不完全理解.
研究的目的:
- 识别细胞感知和响应三细胞结点上的机械张力的分子机制.
- 阐明 Abl 铁酶和 Canoe/Afadin 在上皮细胞机械传导中的作用.
主要方法:
- 在 *Drosophila* 胚胎中研究了机械传导.
- 使用基因操纵来研究Abl激酶和Canoe/Afadin的作用.
- 检查了三细胞结点的蛋白质局部化和酸化动态.
- 评估了Canoe功能改变对细胞粘附和上皮重塑的影响.
主要成果:
- 确定了一条涉及Abl 氨酸激酶和Canoe/Afadin的途径,该途径在三细胞结点的紧张状态下稳定细胞粘附.
- 证实了三细胞结点的Canoe招募是由actomyosin收缩性触发的.
- 表明的Abl依赖化对其机械敏感性至关重要.
- 发现受损的化会破坏三细胞粘附的稳定性,
结论:
- 在上皮重塑过程中发现了一种新型的应力机制,调节三细胞粘附稳定性.
- 卡诺依赖酸化作为一个关键的传感器和传感器的机械张力.
- 这一途径对于维持上皮完整性和使细胞动态重组至关重要.
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