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同性和异性合行为在小鼠的明显下丘脑控制
Tomomi Karigo1, Ann Kennedy1,2, Bin Yang1
1Division of Biology and Biological Engineering, TianQiao and Chrissy Chen Institute for Neuroscience, California Institute of Technology, Pasadena, CA, USA.
Nature
|December 3, 2020
概括
雄性小鼠表现出不同的神经通路,以雌性 (繁殖) 和雄性 (攻击性) 进行安装,通过超声波发声 (USV) 来区分. 这项研究使用特定的下丘脑神经元群解码复杂的行为.
科学领域:
- 神经科学
- 动物的行为
- 内分泌学
背景情况:
- 类似的动物行为可以掩盖不同的潜在意图.
- 区分这些意图的神经基础, 比如雄性小鼠的上升行为, 仍然不清楚.
- 雄性小鼠表现出对雌性和雄性的增加,背景不同.
研究的目的:
- 调查雄性小鼠中雌性和雄性导向的安装是否由不同的神经基质介导.
- 识别涉及这些不同行为的特定的下丘脑神经元群体.
- 了解神经活动模式如何编码不同的行为意图.
主要方法:
- 使用中介前视区域 (MPOA) 和中腹下垂体 (VMHvl) 的雌激素受体1 (ESR1) 阳性神经元的微内镜成像.
- 使用特定神经元亚群的光遗传刺激 (MPOA ESR1VGAT和VMHvl ESR1神经元).
- 分析了超声波发声 (USV) 以区分安装行为 (USV+与USV-).
主要成果:
- 在MPOA和VMHv1中,神经元活动的不同模式使USV+ (女性导向的,生殖性) 和USV- (男性导向的,攻击性) 的接有所差异.
- 对MPOA神经元的光遗传刺激促进了USV+的安装,并将男性导向的攻击转换为USV的安装.
- 刺激VMHvl神经元促进了USV的安装和抑制女性尿液引起的USV,这表明MPOA-VMHvl的相互抑制.
结论:
- 鉴定出不同的下丘脑神经元群体 (MPOA ESR1VGAT和VMHvl ESR1),可因果诱导特定的男性生殖和攻击性行为状态.
- 在MPOA和VMHvl中,繁殖和侵略状态由不同的种群代码编码.
- 具有不同意图的相似行为是由不同的下丘脑神经元群体代表的,突出显示了行为中的神经灵活性.
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