光合作用反应中心内的超快速结构变化
Robert Dods1, Petra Båth1, Dmitry Morozov2
1Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden.
Nature
|December 3, 2020
概括
光合作用反应中心通过通过膜移动电子来使用光能. 这项研究揭示了蛋白质运动如何稳定这些关键的电荷分离步骤.
科学领域:
- 生物化学
- 结构生物学
- 光合作用研究
背景情况:
- 光合作用反应中心对于将光能转化为化学能量至关重要.
- 通过生物膜传输电子是光合作用的一个基本过程.
研究的目的:
- 观察Blastochloris viridis光合作用反应中心的光诱导结构变化.
- 阐明蛋白质动态在电子转移过程中稳定电荷分离的作用.
主要方法:
- 使用X射线自由电子激光器进行时间解析的连续秒晶体学.
- 在皮科秒时间尺度上观察结构变化.
主要成果:
- 特殊双分子的光氧化会引发结构性扰动.
- 电子转移到menaquinone受体导致辅因子运动和蛋白质重组.
- 观察到一种稳定电荷分离的形态动态.
结论:
- 蛋白质利用形态动态来稳定光合作用电子转移的关键步骤.
- 在光合作用反应中心的超快速过程中获得了结构洞察力.
更多相关视频
08:40Separation of Spinach Thylakoid Protein Complexes by Native Green Gel Electrophoresis and Band Characterization using Time-Correlated Single Photon Counting
Published on: February 14, 2019
8.5K
08:04A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry
Published on: March 13, 2014
12.5K
相关概念视频
The Photochemical Reaction Center
4.8K
Reaction centers are pigment-protein complexes that initiate energy conversion from photons to chemical entities. Therefore, photochemical reaction center is a more appropriate term that describes these complexes. The Nobel laureates Robert Emerson and William Arnold provided the first experimental evidence of photochemical reaction centers by demonstrating the participation of nearly 2,500 chlorophyll molecules for the release of just one molecule of oxygen. Despite thousands of photosynthetic...
4.8K
Photosystem I
68.6K
Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...
68.6K
The Antenna Complex
7.2K
Plants and other photosynthetic organisms comprise pigments capable of absorption of direct sunlight. These pigments are present in the reaction center - the main site of photochemical reactions as well as in the antenna complex. Under average light conditions, the rate at which reaction center pigments absorb light is far below the electron transport chain's capacity. As a result, the reaction center alone cannot provide enough energy to drive photosynthesis. The photosynthetic efficiency can...
7.2K
Photosystem II
77.4K
The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...
77.4K
Photosystems
6.4K
Photosystems are multiprotein complexes that form the functional units of photosynthesis in plants, algae, and cyanobacteria. They are found embedded in the membrane of tiny sac-like structures called thylakoids placed inside the chloroplast.
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
Functioning of Photosystems
Photosystems contain many pigment molecules, such as chlorophylls and carotenoids, arranged in a particular organization across two domains — the antenna complex and the reaction center. The main aim of the pigment...
6.4K
The Z-Scheme of Electron Transport in Photosynthesis
12.1K
The light reactions of photosynthesis assume a linear flow of electrons from water to NADP+. During this process, light energy drives the splitting of water molecules to produce oxygen. However, oxidation of water molecules is a thermodynamically unfavorable reaction and requires a strong oxidizing agent. This is accomplished by the first product of light reactions: oxidized P680 (or P680+), the most powerful oxidizing agent known in biology. The oxidized P680 that acquires an electron from the...
12.1K
