Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Proofreading01:31

Proofreading

7.9K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
7.9K
Proofreading01:43

Proofreading

58.5K
Overview
58.5K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

10.6K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
10.6K
PCR01:32

PCR

234.8K
Overview
234.8K
The Replisome03:01

The Replisome

37.3K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
37.3K
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

59.0K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
59.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

An improved guanidine protecting group for the efficient synthesis of GuNA[<i>t</i>-Bu]-modified antisense oligonucleotides.

Organic & biomolecular chemistry·2026
Same author

Synthesis of Peptide-Oligonucleotide Conjugates via Condensation of a Lysine Side-Chain with a Thioester.

The Journal of organic chemistry·2026
Same author

Amidation of Phosphonoacetate-Modified Oligonucleotides and Evaluation of the Properties of the Synthesized Oligonucleotides.

Organic letters·2026
Same author

Development of ARL4C antisense oligonucleotide with reduced off-target effects and enhanced efficacy as an anti-cancer drug.

In vitro cellular & developmental biology. Animal·2026
Same author

Broad-spectrum antiviral activity of antisense oligonucleotides targeting GBF1 against SARS-CoV-2 and influenza viruses.

iScience·2026
Same author

Genetic Encoding of a Trifunctional Photo-Cross-Linker with a Cleavable Alkyl Ester Moiety.

Chembiochem : a European journal of chemical biology·2026

相关实验视频

Updated: Nov 26, 2025

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
07:38

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

14.4K

用于编码和解码锁定核酸序列的高进程性和精度的DNA聚合酶变体

Hidekazu Hoshino1,2, Yuuya Kasahara1,2, Masayasu Kuwahara3

  • 1National Institutes of Biomedical Innovation, Health and Nutrition, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.

Journal of the American Chemical Society
|December 11, 2020
PubMed
概括

研究人员设计了KOD DNA聚合酶变体,以有效合成和转录锁定核酸 (LNA),这是一个有前途的治疗分子. 这些工程聚合酶使得创建新型LNA体和催化剂成为可能,进步了核酸疗法.

更多相关视频

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.6K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.3K

相关实验视频

Last Updated: Nov 26, 2025

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis
07:38

DNA Polymerase Activity Assay Using Near-infrared Fluorescent Labeled DNA Visualized by Acrylamide Gel Electrophoresis

Published on: October 6, 2017

14.4K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

4.6K
Author Spotlight: Advancements in DNA Nanosensors &#8211; Addressing Sensitivity and Selectivity Challenges in Molecular Detection
07:16

Author Spotlight: Advancements in DNA Nanosensors – Addressing Sensitivity and Selectivity Challenges in Molecular Detection

Published on: February 9, 2024

1.3K

科学领域:

  • 生物化学
  • 分子生物学
  • 生物技术

背景情况:

  • 异生物核酸 (XNA) 是DNA和RNA的化学修饰类型,具有治疗潜力.
  • 锁定核酸 (LNA) 是一个有前途的XNA,但由于缺乏高效的合成和反转录工具,其治疗发展受到阻碍.
  • 现有的LNA胺和催化剂开发方法需要专门的聚合酶来进行DNA-LNA合成和LNA-DNA逆转录.

研究的目的:

  • 开发能够有效合成LNA (编码) 和逆转录 (解码) 的工程DNA聚合酶.
  • 提高KODDNA聚合酶的性能,以制造基于LNA的治疗方法.
  • 为了能够合成长的LNA序列,并适应2'-O-甲基 (2'-OMe) 等常见的治疗修饰.

主要方法:

  • 对KODDNA聚合酶进行结构分析以指导蛋白质工程.
  • 局部定向的突变产生LNA解码和编码KOD DNA聚合酶的变体.
  • 工程聚合酶的效率,准确性和基质接受性的实验验证,包括基基长度LNA和LNA合物的SELEX合成.

主要成果:

  • 两种KOD DNA聚合酶变体,KOD DGLNK (DNA → LNA) 和KOD DLK (LNA → DNA) 已成功设计.
  • 这两种变体在LNA合成和逆转录方面显著提高了效率和准确性.
  • 使用KOD DGLNK合成了千基基长的LNA,这些变体接受了2'-O-甲基修饰,这对于治疗应用至关重要.

结论:

  • 工程 KOD DNA 聚合酶变体为 LNA 合成和逆转录提供了强大的工具.
  • 这些变体克服了先前的局限性,促进了新型LNA体和催化剂的开发.
  • 开发的技术通过使自然DNA和RNA不同分子的创建,推动了XNA治疗领域的发展.