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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

3.9K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Nov 25, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

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微生物单细胞RNA测序通过分组条形码

Anna Kuchina1, Leandra M Brettner2,3, Luana Paleologu4,5

  • 1Department of Electrical and Computer Engineering, University of Washington, Seattle, WA, USA.

Science (New York, N.Y.)
|December 18, 2020
PubMed
概括
此摘要是机器生成的。

我们开发了一种新的单细胞RNA测序方法, 这种技术解决了微生物群落中的基因表达异质性,使细菌转录状态的详细分析成为可能.

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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相关实验视频

Last Updated: Nov 25, 2025

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

Published on: May 23, 2018

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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues

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科学领域:

  • 微生物学
  • 基因组学
  • 分子生物学

背景情况:

  • 单细胞RNA测序 (scRNA-seq) 对于真核生物基因表达分析至关重要.
  • 现有的scRNA-seq方法不适合细菌细胞.
  • 了解细菌转录异质性对于微生物学来说至关重要.

研究的目的:

  • 引入microSPLiT (微生物分池结合转录组学),用于细菌的高通量scRNA-seq方法.
  • 为了使细菌中的异质转录状态得到解决.
  • 为细菌群体创建一个全面的基因表达图谱.

主要方法:

  • 开发微SPLiT,一种新型的高通量scRNA-seq技术.
  • 在不同生长阶段对超过25000个 Bacillus subtilis 细胞施用 microSPLiT.
  • 在单细胞水平上分析基因表达特征.

主要成果:

  • 在克拉姆阴性和克拉姆阳性细菌中成功应用microSPLiT.
  • 产生细致的细菌基因表达图谱,揭示了新陈代谢和生活方式的变化.
  • 识别了已知的罕见状态,如能力和传导诱导,以及新的异质基因表达状态.

结论:

  • 微SPLiT是一种高通量单细胞基因表达分析的强大工具.
  • 该方法允许研究以前无法获得的细菌转录异质性.
  • MicroSPLiT为分析复杂的细菌群体,包括自然微生物群开辟了新的途径.