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TMEM41B 是泛病毒宿主因子

H-Heinrich Hoffmann¹, William M Schneider¹, Kathryn Rozen-Gagnon¹

¹Laboratory of Virology and Infectious Disease, The Rockefeller University, New York, NY, USA.

|December 18, 2020

概括

科学家认为TMEM41B是flavivirus复制的关键宿主因素. 这种蛋白质对于病毒RNA复制至关重要,它中的遗传变异可以降低感染率.

科学领域:

病毒学
分子生物学
遗传学

背景情况:

弗拉维病毒是全球重要的健康问题,通过蚊子和咬引起疫情.
识别宿主因素对于理解和控制病毒感染至关重要.

研究的目的:

为了识别菌复制所必需的宿主因素.
描述TMEM41B在病毒生命周期中的作用.

主要方法:

使用全基因组CRISPR-Cas9功能丧失查.
进行了机制研究以阐明TMEM41B的功能.

主要成果:

所有经过测试的Flaviviridae家族成员以及SARS-CoV-2都需要使用TMEM41B.
TMEM41B中的单核酸多态与东亚人群的黄病毒感染减少有关.
在病毒RNA复制复合体中,TMEM41B促进了膜曲率.

结论:

TMEM41B是复制多个病毒家族的关键宿主因素,包括Flaviviridae和Coronaviridae.
TMEM41B在膜曲率中的作用是为病毒基因组复制创造一个受保护的环境的关键.
在TMEM41B的遗传变异对宿主易感性有影响.

关键词:

关于CRISPR的研究其他:在VMP1 自食症冠状病毒黄病毒病毒复制复合物膜改造

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