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相关概念视频

Transcytosis of IgG01:15

Transcytosis of IgG

Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
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The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
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Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen  forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
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循环挤出介于RAG扫描的生理性IGH位置收缩

Hai-Qiang Dai1,2, Hongli Hu3,4, Jiangman Lou3,4

  • 1Howard Hughes Medical Institute, Program in Cellular and Molecular Medicine, Boston Children's Hospital, Boston, MA, USA. hai-qiang.dai@childrens.harvard.edu.

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概括

在原始B细胞中研究RAG扫描显示,位点收缩独立于V(D) J重组. 这一过程由凝聚素介导的循环挤出和CTCF结合元件调节,影响DNA的可访问性.

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科学领域:

  • 免疫学
  • 分子生物学
  • 遗传学

背景情况:

  • RAG内核酶通过扫描染色体以检测重组信号序列 (RSS) 来启动Igh V(D) J重组.
  • 凝聚素介导的循环挤出促进了远程RAG扫描,而CTCF结合元件 (CBE) 可能会阻碍这一过程.
  • 在原始B细胞中,V(H) 位经历了鲜为人知的收缩,这可能会改善远端V(H) 的可访问性.

研究的目的:

  • 阐明V(H) 位置收缩背后的机制及其对RAG扫描的影响.
  • 研究循环挤出和CBEs在原生B细胞中调节V(D) J重组中的作用.

主要方法:

  • 在小鼠原始原生B细胞中分析V(H) 位点逆转.
  • 评估V(H) -RSS和神秘的RSS重排.
  • 评估RAG扫描超出V (H) 的位置.
  • 研究翅膀分离样蛋白同类物 (WAPL) 的表达和耗尽.

主要成果:

  • 尽管局部收缩,但V ((H) 位逆转取消了V ((H) -RSS和通常收的加密RSS重排.
  • 逆转激活了对立方向的加密VH-RSS和RAG扫描超出VH位置.
  • 初级 B 细胞的低 WAPL 表达与局部收缩和 RAG 扫描相关; WAPL 枯竭激活了这些过程.

结论:

  • 通过循环挤出在原始B细胞中,CBE阻碍的放松促进RAG扫描V(H) 位点.
  • 循环挤出涉及V(H) 位置收缩,WAPL影响凝聚力学.
  • 这些发现提供了对V (H) 位点可访问性和V (D) J重组调节的机制性见解.