塑体R6K中的增强剂起源相互作用涉及由启动蛋白介导的DNA循环
S Mukherjee1, H Erickson, D Bastia
1Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
Cell
|February 12, 1988
概括
在R6K等离子体上启动DNA复制.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- 等离子体R6K DNA复制的启动需要特定的起源序列.
- 单独的oriβ序列不足以启动复制.
研究的目的:
- 研究R6K等离子体复制中启动蛋白结合和DNA循环的机制.
- 阐明奥里玛序列在促进奥里β功能中的作用.
主要方法:
- 循环化增强试验试验
- 外核酶III保护测定试验
- 电子显微镜的电子显微镜
- 化学足迹 化学足迹 化学足迹
主要成果:
- 启动蛋白与ori gamma的结合通过长距离的合作性增强了与ori beta的结合.
- 蛋白质介导的ori gamma和ori beta之间的相互作用形成了一个DNA循环.
- 通过多种生物化学和生物物理技术证实了DNA循环的证据.
结论:
- 由启动蛋白结合介导的DNA循环是R6K等离子体DNA复制启动的关键早期步骤.
- 奥里玛序列在cis中起作用,通过蛋白质介导的DNA循环在奥里贝塔促进复制启动.
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