宿主糖酸是细胞内感染期间沙门氏菌毒性的激活信号
Gili Rosenberg1, Dror Yehezkel1, Dotan Hoffman1
1Department of Biological Regulation, Weizmann Institute of Science, 7610001 Rehovot, Israel.
概括
细胞内沙门氏菌感知到了巨细胞的酸盐积累,从而增强了其抗药性和毒性. 这种细菌适应突显了病原体生存的代谢宿主-病原体交谈.
科学领域:
- 微生物学
- 免疫学
- 代谢工程
背景情况:
- 细胞内病原体必须适应宿主细胞环境.
- 巨细胞的新陈代谢程序会导致对微生物的炎症反应.
- 这种重编程在细菌适应中的作用尚不清楚.
研究的目的:
- 研究细菌病原体如何适应细胞内环境.
- 探索巨代谢重编程在细菌生存和毒性的作用.
主要方法:
- 代谢分析
- 双RNA测序
- 对沙门氏菌 (S. Tm) 的基因操纵
主要成果:
- 巨细胞中的酸盐积累被细胞内S. Tm感知.
- 用于增强抗菌素耐药性和III型分泌.
- 在巨和小鼠中,缺乏酸转运体DcuB的STM显示了生存率的降低.
结论:
- 沙门氏菌 Typhimurium 通过巨细胞的新陈代谢编程来实现自身的毒性和生存.
- 酸盐作为巨细胞内的细菌适应的关键信号.
- 这项研究提供了对宿主-病原体代谢相互作用的新见解.
更多相关视频
11:10High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
Published on: August 11, 2014
13.0K
10:57NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells
Published on: January 12, 2020
10.9K
相关概念视频
Bacterial Signaling
38.6K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
38.6K
Gene Regulation in Microbial Communities: Quorum Sensing
255
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
255
