时钟基因和环境线索协调虫的激素合成,蜂群和交配
Guandong Wang1,2, Joel Vega-Rodríguez3, Abdoulaye Diabate4
1CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences (CAS), Shanghai, China.
Science (New York, N.Y.)
|January 22, 2021
概括
蚊子的交配和蜂群是由内在的时钟基因,光线和温度控制的. 破坏时钟基因的周期性 (per) 和无时间性 (tim) 显著影响了Anopheles蚊子的交配行为.
科学领域:
- 昆虫学
- 时间生物学
- 分子生物学
背景情况:
- 蚊子的交配方式是雄性蚊子在黄昏时群聚,
- 众所周知,光线和温度等环境因素会影响昆虫的行为,包括交配.
研究的目的:
- 调查时钟基因,光线和温度在协调蚊子和交配行为中的作用.
- 确定这些行为背后的特定基因和分子机制.
主要方法:
- 对野外捕获的雄性鱼的转录组分析.
- 在实验室和半场环境中对周期性 (per) 和永恒性 (tim) 基因进行基因淘汰实验.
- 脱酶1 (desat1) 基因表达的分析及其在皮质碳化合物生产中的作用.
主要成果:
- 在蜂群的Anopheles coluzzii雄的头部上调时钟基因周期 (per) 和无时代 (tim).
- 在实验室中,per和tim的击落显著降低了Anopheles gambiae s.s.和Anopheles stephensi的交配成功. 在半场条件下群聚/交配.
- 在成群的雄性中,脱酶1 (desat1) 被上调并以节奏方式表达,调节刺激交配的状碳水化合物,如甲.
结论:
- 时间基因,光线和温度是蚊子繁殖和交配的关键调节者.
- 经期和无时代基因对于雄性交配和群体行为至关重要.
- 通过desat1介导的特定的皮质碳化合物在刺激蚊子交配方面发挥着作用.
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