古老的平衡选择维持了酵母体中不可兼容的银河糖路径
James Boocock1,2,3,4, Meru J Sadhu1,2,3,4, Arun Durvasula1
1Department of Human Genetics, University of California, Los Angeles, Los Angeles, CA, USA.
概括
数百万年来,通过平衡选择来维持酵母银河糖代谢途径的两个不相容版本. 这种涉及GAL2,GAL1/10/7和PGM1基因的遗传变异显示了银河糖和葡萄糖利用之间的权衡.
科学领域:
- * 进化生物学
- * 酵母遗传学
- * 代谢途径的演变
背景情况:
- * 代谢途径通常在物种内保持.
- * 代谢途径的变化可能导致功能差异.
- 在Saccharomyces cerevisiae中,银河糖利用途径对银河糖生长至关重要.
研究的目的:
- * 调查酵母银河糖路的功能变异的遗传基础.
- * 了解不兼容的路径版本的进化维护.
- * 确定导致这种变化的选择性压力.
主要方法:
- * 确定影响银河糖生长的三位基因相互作用.
- *为*GAL2*,*GAL1/10/7*和*PGM1*基因设计特定的等位基因.
- 在不同碳来源中对工程酵母菌株进行表型分析.
主要成果:
- 在Saccharomyces cerevisiae中发现了两个功能不相容的银河糖路.
- * 证明不兼容性来自于*GAL2*,*GAL1/10/7*和*PGM1*的特定基因.
- 证明平衡选择已经维持了这些不相容的等位基因数百万年.
结论:
- * 多部位平衡选择保持了银河糖路的不兼容版本.
- * 替代基因在乳制品环境中普遍存在,并提供增长权衡.
- 这种权衡可能是长期平衡选择的基础.
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