相关实验视频

Updated: Nov 19, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

扩展测序:完整的生物系统中的空间精确的转录组学

Shahar Alon^1,2,3, Daniel R Goodwin^1,2, Anubhav Sinha^1,2,4

¹Department of Media Arts and Sciences, MIT, Cambridge, MA, USA.

Science (New York, N.Y.)

|January 29, 2021

概括

扩展测序 (ExSeq) 提高了RNA成像分辨率,使得基因表达的纳米尺度映射成为可能. 这种技术透露了各种组织和细胞类型的详细RNA定位.

科学领域:

分子生物学
神经科学
基因组学

背景情况:

目前的多重复合RNA成像缺乏纳米级分辨率,限制了转录本地化到亚细胞区.
准确的空间转录学对于理解细胞功能和疾病至关重要.

研究的目的:

适应扩展显微镜以纳米级分辨率进行现场RNA测序.
开发一种从亚细胞到系统尺度适用的高度多重化的长读RNA测序方法.

主要方法:

适应扩展显微镜用于非目标和目标在位RNA测序.
应用扩展测序 (ExSeq) 进行小鼠大脑,海马和人类转移性乳腺癌活检.

主要成果:

在小鼠大脑中提供了成千上万的基因读数,
向ExSeq在海马神经元中生成纳米级RNA地图,揭示了树突和脊柱水平的转录本地化.
ExSeq 在视觉皮层中确定了细胞类型和层特定的RNA模式,并在人类癌症活检中绘制了瘤和免疫细胞.

结论:

扩展测序 (ExSeq) 克服了RNA成像中的分辨率限制.
ExSeq能够从纳米尺度到系统尺度进行高度多重的RNA映射.
这种技术为各种生物系统的空间转录学提供了前所未有的洞察力.

更多相关视频

In Situ Hybridization for the Precise Localization of Transcripts in Plants

In Situ Hybridization for the Precise Localization of Transcripts in Plants

Published on: November 23, 2011

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

Multiplex Detection of Gene Expression in the Intact Drosophila Brain Using Expansion-Assisted Iterative Fluorescence In Situ Hybridization

Published on: May 2, 2025

相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...