用单细胞蛋白组学进行细胞周期的时空剖析
Diana Mahdessian1, Anthony J Cesnik1,2,3, Christian Gnann1,3
1Science for Life Laboratory, School of Engineering Sciences in Chemistry, Biotechnology and Health, KTH - Royal Institute of Technology, Stockholm, Sweden.
Nature
|February 25, 2021
概括
这项研究绘制了细胞周期中的人类蛋白质异质性,揭示了与癌症相关的细胞对细胞蛋白质变异. 大多数蛋白质变化发生在翻译后,而不是通过基因表达,影响细胞命运和新陈代谢.
科学领域:
- 细胞生物学
- 蛋白质组学
- 基因组学
背景情况:
- 细胞循环控制细胞生长和分裂, 这对生命至关重要.
- 细胞循环的失调与癌症等疾病有关.
- 现有的研究缺乏细胞对细胞蛋白质变异性的系统分析.
研究的目的:
- 创建一个全面的时间空间地图的人类蛋白质异质.
- 在细胞周期中研究蛋白质水平的细胞间变异性.
- 识别与线粒分裂相关的蛋白质及其潜在的致癌功能.
主要方法:
- 在亚细胞分辨率上整合蛋白质组与单细胞转录组.
- 在细胞周期内精确测量单个细胞的时间.
- 蛋白质组和转录组数据的分析,以确定细胞间的变异性.
主要成果:
- 大约20%的人类蛋白质组表现出细胞间的变异性.
- 发现了数百种与细胞分裂和细胞循环有关联的新型蛋白质.
- 细胞循环的进展占观察到的细胞间变异性的不到一半;大多数蛋白质都是通过翻译后调节的.
- 在循环和非循环变量蛋白之间,激酶介导的酸化不同,影响细胞命运和新陈代谢.
结论:
- 这项研究提供了细胞周期的详细蛋白质图,突出显示了细胞间的显著异质性.
- 鉴定出具有潜在致癌功能的蛋白质需要在癌症研究中进行进一步的研究.
- 这些发现强调了在细胞循环期间调节蛋白质水平的翻译后修改的重要性.
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