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通过电荷检测质谱测量揭示了三元型SARS-CoV-2尖端蛋白质上的甘氨酸处理异质性

Lohra M Miller¹, Lauren F Barnes¹, Shannon A Raab¹

¹Chemistry Department, Indiana University, 800 E Kirkwood Ave, Bloomington Indiana 47405, United States.

Journal of the American Chemical Society

|March 3, 2021

概括

电荷检测质谱显示了SARS-CoV-2尖端蛋白质糖化中的显著异质性. 这一发现表明糖质量比以前理解的更广泛,可能有助于免疫逃避.

科学领域:

生物化学
结构生物学
病毒学

背景情况:

SARS-CoV-2 尖端 (S) 蛋白质具有很强的糖化,每个三元体有 66 个 N-糖位.
在病毒进入,免疫逃避和疫苗开发中,糖化起着至关重要的作用.
了解糖化异质性对于开发有效的治疗方法至关重要.

研究的目的:

评估SARS-CoV-2尖端 (S) 蛋白三元体上的N结合糖化异质性.
通过电荷检测质谱法 (CDMS) 获得的糖质量分布与现有的糖蛋白组学数据进行比较.
调查观察到的糖化异质性对病毒免疫规避策略的影响.

主要方法:

利用电荷检测质谱法 (CDMS) 对个别SARS-CoV-2S蛋白质剪切器进行"上下"分析.
同时测量单个离子的质量与电荷比,以确定质量分布.
将CDMS衍生的糖质量数据与"自下而上的"糖蛋白组学研究的结果进行比较.

主要成果:

与糖蛋白组学研究相比,CDMS显示平均糖质量显著增加 (35-47%的增加).
糖蛋白组"自下而上的"方法似乎低估了更大,更复杂的糖的丰富性.
CDMS显示了比预期的更广泛的糖质量分布,这表明S蛋白修剪剂中的糖处理具有显著的异质性.
观察到的异质性表明单个trimers上的差异性糖处理,而不是统一的处理.

结论:

在SARS-CoV-2 S蛋白质糖化过程中,糖质质量和加工过程呈现出相当大的异质性.
与传统的糖蛋白组学相比,CDMS提供了更全面的糖复杂性.
这种广泛的糖化异质性可能是病毒逃避宿主免疫系统的机制.
对S蛋白糖化进行进一步的研究对于了解病毒病变和告知治疗策略至关重要.

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