TOR-EIN2轴调节核信号以调节植物生长
Liwen Fu1,2, Yanlin Liu2, Guochen Qin1
1Shanghai Centre for Plant Stress Biology, Chinese Academy of Science Centre for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, P. R. China.
Nature
|March 4, 2021
概括
拉巴素 (TOR) 激酶的目标直接化乙烯不敏感蛋白2 (EIN2),调节基因表达和细胞生长. 这揭示了TOR如何控制植物的全球转录的新机制.
科学领域:
- 植物分子生物学
- 细胞信号通路
- 单核细胞生长调节
背景情况:
- 拉巴素 (TOR) 激酶的标是细胞生长和增殖的保护性主调节剂,可以整合各种环境信号.
- 虽然TOR在翻译中的作用已经得到了充分的研究,但它对全球转录网络的控制在很大程度上是未知的.
研究的目的:
- 研究乙烯不敏感蛋白2 (EIN2) 作为Arabidopsis thaliana中的TOR直接基质的作用.
- 阐明TOR信号通过EIN2调节全球转录的机制.
主要方法:
- 确定EIN2是葡萄糖激活的TOR激酶的直接基质.
- 对ein2-5突变的分析,以评估对TOR导向的转录重编程的影响.
- 使用化学,细胞和遗传分析来剖析信号通路.
主要成果:
- 通过葡萄糖激活的TOR直接化EIN2,抑制其核定位.
- 艾因2-5突变体表现出受损的葡萄糖-TOR导向的全球转录编程.
- EIN2对涉及DNA复制,细胞壁和脂质合成以及二次代谢的广泛的TOR基因进行负面调节.
- 由葡萄糖-TOR-EIN2轴控制的细胞延长和增殖与乙烯信号通路不同.
结论:
- TOR直接化了EIN2,揭示了TOR对全球转录的新机制.
- 葡萄糖-TOR-EIN2信号轴控制细胞生长和增殖独立于标准的乙烯信号.
- 在EIN2上不同的酸化代码介导其整合到不同的信号通路中,突出显示了多功能信号枢纽.
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