Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

9.9K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
9.9K
Factors Influencing Microbial Growth: Temperature01:27

Factors Influencing Microbial Growth: Temperature

693
Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
693
Diversity of Archaea IV01:29

Diversity of Archaea IV

255
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
255
Effect of Temperature Change on Reaction Rate02:28

Effect of Temperature Change on Reaction Rate

4.6K
The Arrhenius equation,
4.6K
Temperature Dependence on Reaction Rate02:55

Temperature Dependence on Reaction Rate

85.5K
The Collision Theory
Atoms, molecules, or ions must collide before they can react with each other. Atoms must be close together to form chemical bonds. This premise is the basis for a theory that explains many observations regarding chemical kinetics, including factors affecting reaction rates.
The collision theory is based on the postulates that (i) the reaction rate is proportional to the rate of reactant collisions, (ii) the reacting species collide in an orientation allowing contact between...
85.5K
Effects of Temperature on Free Energy02:11

Effects of Temperature on Free Energy

27.1K
The spontaneity of a process depends upon the temperature of the system. Phase transitions, for example, will proceed spontaneously in one direction or the other depending upon the temperature of the substance in question. Likewise, some chemical reactions can also exhibit temperature-dependent spontaneities. To illustrate this concept, the equation relating free energy change to the enthalpy and entropy changes for the process is considered:
27.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Perspective on a challenge: Predicting the photochemistry of cyclobutanone.

The Journal of chemical physics·2026
Same author

Optimal 1TEL-target protein linker character is target protein-dependent. Corrigendum.

Acta crystallographica. Section D, Structural biology·2026
Same author

First-Principles Analysis of Protonation-Induced Electronic Effects in Tetrakis(<i>p</i>-aminophenyl)porphyrin (TAPP).

The journal of physical chemistry. A·2026
Same author

Probing the Ultrafast Photodynamics of Dihydroazulene with In Silico Time-Resolved Photoelectron Spectroscopy and Ultrafast Electron Diffraction.

The journal of physical chemistry. A·2026
Same author

Large field of view fluorescence imaging of microfluidic devices with a tandem-lens macroscope.

Lab on a chip·2026
Same author

Mechanistic Understanding of Protein-MOF Integration Through Surfactant-Driven Interfacial Design.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

相关实验视频

Updated: Nov 15, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.3K

酶温度适应的并行分子机制

Margaux M Pinney1, Daniel A Mokhtari2, Eyal Akiva3

  • 1Department of Biochemistry, Stanford University, Stanford, CA 94305, USA. margauxp@stanford.edu herschla@stanford.edu.

Science (New York, N.Y.)
|March 6, 2021
PubMed
概括

酶适应温度对于分子进化至关重要,通常是单个氨基酸变化的结果. 这项研究揭示了细菌酶的广泛并行进化,

更多相关视频

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays
03:09

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays

Published on: August 9, 2024

1.0K
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.2K

相关实验视频

Last Updated: Nov 15, 2025

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes
13:30

A New Screening Method for the Directed Evolution of Thermostable Bacteriolytic Enzymes

Published on: November 7, 2012

18.3K
Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays
03:09

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays

Published on: August 9, 2024

1.0K
Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes
09:42

Unraveling Entropic Rate Acceleration Induced by Solvent Dynamics in Membrane Enzymes

Published on: January 16, 2016

9.2K

科学领域:

  • 分子进化
  • 酵素学
  • 生物物理

背景情况:

  • 了解酶适应温度是分子进化的关键.
  • 酶必须在不同温度下保持活性和稳定性.
  • 温度适应的进化策略是多样化的.

研究的目的:

  • 研究酶温度适应的分子和进化机制.
  • 将机理学研究与大规模序列分析结合起来.
  • 确定温度适应的关键残留物和进化模式.

主要方法:

  • 对类异构酶 (KSI) 的深度机制研究.
  • 对数千种细菌酶进行了全面的序列分析.
  • 对残留物质,分子相互作用和网络的评估.

主要成果:

  • 在KSI中,温度适应是由单个残留物变化和最小的表皮变化驱动的.
  • 这种适应机制在各种KSI背景中观察到,表明并行进化.
  • 在1005个细菌酶家族中发现与生物体生长温度相关的残留物,表明广泛的并行适应.

结论:

  • 单个残留物变化是酶温度适应的重要驱动因素.
  • 并行进化是细菌酶适应温度的一个常见策略.
  • 具体的残留物质和相互作用是温度适应的基础.