相关实验视频
Updated: Aug 15, 2026

17:27
Preparation of Aplysia Sensory-motor Neuronal Cell Cultures
Published on: June 8, 2009
在Aplysia中长期的异质突触抑制
P G Montarolo1, E R Kandel, S Schacher
1Howard Hughes Medical Institute, Columbia University, College of Physicians and Surgeons, New York, New York.
Nature
|May 12, 1988
概括
甲基Phe-Met-Arg-Phe-amide (FMRFamide) 诱导了阿普利西亚的长期突触抑制.
科学领域:
- 神经科学是一个神经科学.
- 细胞神经科学 细胞神经科学
- 突触性可塑性 突触性可塑性
背景情况:
- 在Aplysia的部撤回反射中,突触传递表现出短期和长期调制.
- 短期的突触抑郁,持续数分钟,由Phe-Met-Arg-Phe-amide (FMRFamide) 通过酸氧酶通路诱导.
研究的目的:
- 为了研究诱导长期突触抑郁 (LTD) 在阿普利西亚部撤回反射.
- 确定FMRFamide是否可以诱导LTD,并将其特征与短期抑郁和其他调节途径进行比较.
主要方法:
- 利用Aplysia细胞培养来研究单突触感官与运动的连接.
- 在2小时内多次应用FMRFamide,以诱导长期变化.
- 研究了一种蛋白质合成抑制剂阿尼索米辛对FMRFamide诱导的抑郁症的影响.
主要成果:
- 在2个小时内5次使用FMRFamide诱导了持续24小时的长期突触抑郁.
- FMRFamide诱导的LTD是发射器特定的;多巴胺和低频刺激没有诱导LTD.
- 与短期抑郁症不同的是,长期抑郁症被安尼索米辛阻断,这表明需要蛋白质合成.
结论:
- 在Aplysia中长期的突触抑郁,类似于长期的促进,需要蛋白质合成.
- 异突突触抑郁症,像促进一样,存在于短期和长期的形式,后者依赖于基因产品.
相关概念视频
Long-term Potentiation
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre- and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Long-term Depression
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
Long-term Potentiation
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when presynaptic neurons...
Hebbian LTP
LTP can occur when presynaptic neurons...
Long-term Depression
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
Calcium Ion Concentration Mechanism
If over time, all...
Calcium Ion Concentration Mechanism
If over time, all...
Chemical Synapses
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
Ligand-Gated Ion Channel Receptor: Gating Mechanism
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...

