多级蛋白质组学揭示了SARS-CoV-2和SARS-CoV引起的宿主干扰
Alexey Stukalov1, Virginie Girault1, Vincent Grass1
1Technical University of Munich, School of Medicine, Institute of Virology, Munich, Germany.
Nature
|April 12, 2021
概括
这项研究整合了多组数据,绘制了SARS-CoV-2和SARS-CoV与宿主细胞的相互作用. 这些发现揭示了不同的分子机制,并确定了COVID-19治疗的潜在药物标.
科学领域:
- 病毒学
- 分子生物学
- 系统生物学
背景情况:
- 由于SARS-CoV-2的全球传播,需要了解病毒蛋白的功能和宿主相互作用.
- 之前的OMIC研究提供了COVID-19病理生理学的细分见解.
- 由于实验系统的异质性,整合多种omics数据以全面了解病毒与宿主之间的相互作用是具有挑战性的.
研究的目的:
- 对SARS-CoV-2和SARS-CoV进行并发的多组研究.
- 描述病毒相互作用及其对宿主细胞系统的影响.
- 确定这些冠状病毒的共同和独特的分子机制.
主要方法:
- 对感染SARS-CoV-2和SARS-CoV的人类肺细胞系的同时进行多基因组分析 (蛋白质组,转录基因组,全基因组,蛋白质组).
- 两种病毒的交互分子概况.
- 网络分析将多组数据投射到蜂互动网络上.
主要成果:
- 对SARS-CoV-2和SARS-CoV进行了全面的交互分析.
- 在多个分子层 (转录体,蛋白体,无处不在体,蛋白体) 中分析了病毒诱导的干扰.
- 鉴定了SARS-CoV-2 ORF8对TGF-β通路的特定失调以及SARS-CoV-2 ORF3的自.
- 网络分析显示了SARS-CoV-2和SARS-CoV感染之间的交叉通话和不同的/共同的分子机制.
- 鉴定出具有强效抗病毒作用的潜在药物点,包括酶和矩阵金属蛋白酶抑制剂.
结论:
- 这项研究提供了一个综合的多组数据集,以了解SARS-CoV-2和SARS-CoV的病原性.
- 这些病毒使用的独特和共同的分子机制得到了阐明.
- 这些发现突出了潜在的治疗策略,包括针对特定途径和识别具有抗病毒活性的现有药物.
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