一个氨酸-氨酸FRET分离探头用于血红氧酶-1

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Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
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Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

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Edward R H Walter1,2, Ying Ge2, Justin C Mason2

  • 1Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus, Wood Lane, London W12 0BZ, U.K.

Journal of the American Chemical Society
|April 13, 2021
PubMed
概括

研究人员开发了一种新型的光探测器Fe-L1,用于检测血红素酶-1 (HO-1) 的活性. 这种探测器可以监测与心血管和神经退行性疾病相关的HO-1酶, 提供早期诊断的潜力.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...