相关实验视频

Updated: Nov 9, 2025

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine

Published on: July 28, 2023

人类扩展多能干细胞对子胚胎的化学贡献

Tao Tan¹, Jun Wu², Chenyang Si¹

¹State Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, Yunnan 650500, China.

|April 16, 2021

概括

人类扩展多能干细胞 (hEPSC) 成功地集成到子胚胎中,证明了研究早期人类发育和促进再生医学的潜力. 这项研究探讨了灵长类中跨物种的嵌合体形成.

科学领域:

干细胞生物学
发育生物学
灵长类研究

背景情况:

人类多能干细胞 (hPSC) 对于体内多能性评估和再生医学至关重要.
之前的研究表明hPSC在像小鼠和猪这样的进化远离物种中的贡献有限.
对于移植和发育研究来说, 了解物种间的嵌合体形成是关键.

研究的目的:

在Cynomolgus子胚胎中研究人类扩展多能干细胞 (hEPSC) 的仿真能力.
探索HEPSC在促进灵长类早期发展方面的潜力.
发现涉及跨物种细胞交叉通话的信号机制.

主要方法:

在Cynomolgus子胚胎的生殖过程中.
在子胚胎中引入HEPSC.
细胞存活,增殖和基因系贡献的分析.
研究跨物种的信号通路.

主要成果:

在子胚胎中显示了HEPSC的生存和繁殖.
这些HEPSC有助于多个植入前和早期植入后的细胞系.
确定了影响人类和子细胞发育的信号事件.
建立了一个研究人类与灵长类之间的发展模型.

结论:

在灵长类胚胎中拥有虚构能力,为发育生物学提供了一个新模型.
这些发现为人类早期发展和灵长类动物进化提供了洞察力.
结果可能会为增强进化远端物种的复原医学策略提供信息.

关键词:

活体培养的莫克伊胚胎人类扩展多能干细胞人类和子合体胚胎跨物种的木乃伊多能干细胞

更多相关视频

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Trans-inner Cell Mass Injection of Embryonic Stem Cells Leads to Higher Chimerism Rates

Published on: May 29, 2018

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

Published on: February 15, 2019

相关概念视频

Embryonic Stem Cells

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...