为深层组织检测氧化而定制的NIR-II光声探测器的开发
Melissa Y Lucero1, Amanda K East1, Christopher J Reinhardt1
1Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States.
Journal of the American Chemical Society
|April 27, 2021
概括
研究人员开发了一种新的近红外II (NIR-II) 探测器APNO-1080,用于增强氧化的光声学 (PA) 成像. 这种探测器为深层组织生物医学应用提供了提高的灵敏度和减少的背景干扰.
科学领域:
- 生物医学光学
- 分子成像
- 化学生物学
背景情况:
- 光声学 (PA) 影像是用于疾病查和分析物的宝贵工具.
- 目前使用近红外I (NIR-I) 光的抗氧化剂面临着血红蛋白等内源分子的干扰.
- 近红外II (NIR-II) 波长提供了减少的背景,使得低度目标如氧化 (NO) 的检测成为可能.
研究的目的:
- 开发APNO-1080,用于敏感深层组织PA成像的NIR-II无响应探针.
- 克服NIR-I PA成像的局限性,特别是对低丰度分析物的背景干扰和灵敏性.
主要方法:
- 采用了双相调方法,涉及哈梅特和布伦斯特德分析,以确定基于氨酸的选择性NO触发剂.
- 对NIR-II平台进行选,以选择具有最小聚合的化学结构,保持PA信号完整性.
- 在正位乳腺癌和异位肺癌模型中进行了"体外"幻影测定和"体内"PA成像.
主要成果:
- 与NIR- I类似物相比,APNO- 1080的灵敏度在体外幻影测试中是17. 7倍的.
- 在活体中,PA成像显示在正位乳腺 (1.3 ± 0.12) 和异位肺癌 (1.65 ± 0.07) 模型中显著的正常化激活反应.
结论:
- APNO-1080是一种高度敏感和选择性的NIR-II无反应探针,适用于深层组织PA成像.
- 开发的探测器有效减少背景干扰,并在复杂的生物环境和疾病模型中增强NO的检测.
相关概念视频
IR Spectrometers
There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...
Atomic Absorption Spectroscopy: Instrumentation
An atomic absorption spectrophotometer (AAS) comprises several components: a radiation source, an atomizer, a monochromator, and a detector. The radiation source can be a hollow-cathode lamp (HCL) or an electrodeless-discharge lamp (EDL), both of which provide a narrow emission line of the required wavelength. However, some instruments use continuum sources and high-resolution monochromators to achieve a narrow range of radiation.
The atomizer used in AAS can be either a flame atomizer or an...
The atomizer used in AAS can be either a flame atomizer or an...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used.


