在SARS-CoV-2非RBD尖端表征的流行,保护性和融合性IgG识别
William N Voss1, Yixuan J Hou2, Nicole V Johnson1
1Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX, USA.
概括
在SARS-CoV-2感染后,大多数抗体都准RBD外的尖端蛋白. 这些非RBD抗体,包括针对N终端域 (NTD) 的抗体,普遍存在,对COVID-19变种产生影响.
科学领域:
- 免疫学
- 病毒学
- 蛋白质组学
背景情况:
- 在SARS-CoV-2感染后,免疫球蛋白G (IgG) 抗体的特定点在很大程度上仍未被描述.
- 了解抗体结合部位对于开发针对SARS-CoV-2及其变种的有效疫苗和治疗方法至关重要.
研究的目的:
- 在SARS-CoV-2感染后确定血中IgG抗体的分子组成和结合表位.
- 调查针对SARS-CoV-2尖端蛋白的不同区域的抗体的流行率和特征.
主要方法:
- 在康复期的个体中,IgG抗体对抗SARS-CoV-2尖端糖蛋白的蛋白质分解.
- 已识别的抗体系的遗传,结构和功能特征.
- 与病毒变异相关的抗体反应的分析.
主要成果:
- 大多数 (> 80%) 的IgG反应针对尖端蛋白的受体结合域 (RBD) 外的表位.
- 抗体反应的很大一部分可以归因于少数占优势的IgG基因系,包括那些向N端域 (NTD) 的基因系.
- 一种由"公众"抗体向的复发性NTD表征在令人担忧的SARS-CoV-2变种中经常发生突变.
结论:
- 在SARS-CoV-2感染后的血IgG抗体主要向非RBD表位,特别是NTD.
- 针对"公共"NTD的抗体普遍存在,并在抗病毒保护中发挥作用.
- 常见的NTD表征突变对抗体逃逸和COVID-19疫苗和治疗的有效性有影响.
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