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一个单细胞分辨率的玉米的cis-regulatory图谱

Alexandre P Marand¹, Zongliang Chen², Andrea Gallavotti³

¹Department of Genetics, University of Georgia, Athens, GA 30602, USA.

|May 8, 2021

概括

这项研究绘制了玉米细胞中的 cis 调节元件 (CREs) 和转录因子 (TFs),揭示了它们在基因调节和进化中的作用. 这些发现为植物的发展和育种提供了洞察力,并为更广泛的应用提供了新的软件.

科学领域:

植物基因组学
分子生物学
发育生物学

背景情况:

控制基因表达对于专门的细胞功能至关重要.
了解CREs和转录因子 (TF) 是解读基因调节和细胞多样性的关键.

研究的目的:

通过单细胞基因组学在6个玉米器官中绘制CREs和TFs.
确定定义细胞身份,染色体组织和基因表达的调节因素.
调查玉米和Arabidopsis thaliana之间的CREs和TFs的进化动态.

主要方法:

六个玉米器官的单细胞基因组分析.
对转录因子 (TF) 组合学和非细胞自主活动的分析.
参与高阶染色体相互作用的TF的鉴定.
使用Arabidopsis thaliana进行比较基因组学分析.

主要成果:

细胞类型特异性的CREs为增强剂活性增强,并位于未甲基化的LTR逆转子中.
在玉米繁殖中,CREs是表型相关遗传变异的热点和选择目标.
鉴定了玉米和阿拉比多普西斯之间的保守和分离的TF和CRE动态,揭示了基因调控网络的演变.
开发了一个单细胞分析软件Socrates.

结论:

这份CRE图谱为了解玉米的基因调节和发育提供了基础资源.
在植物的适应和繁殖中,CREs扮演着重要的角色,它们是重要的遗传变体.
比较分析突出了植物基因调节网络的进化可塑性.
苏格拉底软件为跨物种的 cis-regulatory 研究提供了一种多功能工具.

关键词:

ATAC-seq 其他染色体其他监管要素表观遗传学基因调节玉米植物发展虚假时间一个细胞转录系数

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