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纤维细胞系的跨组织组织
Matthew B Buechler1, Rachana N Pradhan1, Akshay T Krishnamurty1
1Genentech, South San Francisco, CA, USA.
Nature
|May 13, 2021
概括
这项研究使用单细胞转录学绘制了多种组织和物种中的纤维细胞图. 它揭示了老鼠和人类保存的纤维细胞亚型, 对于组织结构和疾病至关重要.
科学领域:
- 细胞生物学
- 基因组学
- 组织工程
背景情况:
- 纤维细胞是关键的结构细胞,参与组织结构,平衡,以及纤维化和癌症等疾病过程.
- 最近的研究表明纤维细胞在组织中的异质性,但缺乏跨不同器官和条件的全面的单细胞分辨率图谱.
研究的目的:
- 使用健康和疾病状态的多种组织的单细胞转录组数据创建全面的纤维细胞图谱.
- 识别普遍的纤维细胞亚型,并了解它们在组织平衡和疾病发病中的作用.
- 为了比较物种之间的纤维细胞转录状态,特别是小鼠和人类.
主要方法:
- 在17个组织,50个数据集,11个疾病状态和2个物种中整合了大约23万个纤维细胞的单细胞转录数据.
- 构建小鼠纤维细胞图谱并使用DptIRESCreERT2的小鼠模型.
- 鼠标和人类纤维细胞图谱的比较分析,包括那些来自扰乱状态的图谱.
主要成果:
- 在小鼠组织中保存的两个通用纤维细胞转录亚型的识别.
- 证据表明这些通用纤维细胞在稳定状态和疾病条件下充当专门和激活纤维细胞群的储存库.
- 在小鼠和人类之间展示保存的纤维细胞转录状态,包括通用和疾病相关的激活表型.
结论:
- 一种跨物种,全组织单细胞转录学方法揭示了纤维细胞系的基本组织原理.
- 保存的纤维细胞亚型和状态强调它们在维持组织健康和驱动疾病病理方面的关键作用.
- 这些发现为了解纤维细胞生物学和开发纤维细胞相关疾病的向治疗提供了基础资源.
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