ZAR1抗体是一个透通道,触发植物免疫信号传递
1State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Cell
|May 13, 2021
概括
植物免疫受体ZAR1在等离子体膜中形成一个 pentameric 复合体,即抵抗体. 这种抵抗体作为透性离子通道起作用,触发植物免疫反应和细胞死亡.
科学领域:
- 植物免疫力 植物免疫力
- 植物防御的分子机制
- 离子通道功能 离子通道功能
背景情况:
- 结合核酸,富含白素的重复受体 (NLRs) 是植物和动物中至关重要的免疫传感器.
- 阿拉比多普西斯NLR蛋白ZAR1形成一个抵抗体,启动植物免疫反应和细胞死亡.
研究的目的:
- 为了研究激活ZAR1抗体的体内结构和功能.
- 确定ZAR1抗体作为植物免疫中的离子通道的作用.
主要方法:
- 单分子成像用于可视化ZAR1复合体在等离子体膜.
- 在Xenopus卵细胞和平面脂质双层中的电生理学记录.
- 分析ZAR1介导的Ca2+流入,活性氧物种的产生和植物细胞中的细胞死亡.
主要成果:
- 激活的ZAR1在血膜中形成pentameric复合体.
- ZAR1抗体体表现出透性离子通道活性,这取决于Glu11残留物.
- 在植物中ZAR1的激活会导致Ca2+的流入,ROS的产生和细胞死亡.
结论:
- 该ZAR1抗体组作为透性离子通道的功能.
- 这种通道活动对于ZAR1介导的植物免疫和细胞死亡诱导至关重要.
关键词:
阿拉比多普西斯 (Arabidopsis) 是一种植物.在NLR中,它是NLR.伪omonas 是一个类型的.克桑托蒙纳斯 (Xanthomonas) 是一种通道中的.细胞死亡是细胞死亡.疾病耐药性 疾病耐药性植物免疫力 植物免疫力电阻组对抗体的作用单个分子成像技术更多相关视频
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