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相关概念视频

Recombinant DNA01:09

Recombinant DNA

Overview
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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相关实验视频

Updated: Jul 15, 2026

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

在塑体上最大限度地提高基因表达,使用 in vitro 重组.

K Backman, M Ptashne

    Cell
    |January 1, 1978
    PubMed
    概括

    研究人员使用大肠杆菌中的乳酸促进剂设计了菌体兰巴达抑制剂合成. 这种方法可以实现高水平的基因表达,可能适用于各种基因.

    科学领域:

    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.
    • 生物技术是生物技术.

    背景情况:

    • 菌体兰巴抑制剂 (cl) 调节病毒基因表达.
    • 拉克促销者是Escherichia coli中一个特征很好的强促销者.
    • 细菌中特定基因的有效表达对研究和生物技术至关重要.

    研究的目的:

    • 为了研究使用lac促进剂来驱动lambda抑制剂合成.
    • 为了确定促进物放置是否会影响抑制物基因表达水平.
    • 开发一种方法来实现大肠杆菌中高水平的基因表达.

    主要方法:

    • 在体外再组合技术被用来将LAC促进物与等离子体上的lambda cl基因融合.
    • 不同的结构改变了LAC促进体和CL基因之间的距离.
    • 在携带这些结构的大肠杆菌菌株中分析了基因表达水平.

    主要成果:

    • 兰巴达抑制剂合成在所有构造中都由lac促进剂成功驱动.
    • 一个特定的融合导致了异常高水平的兰巴抑制剂.
    • 这种高表达性融合的特点是,混合的核糖体结合部位具有lambda和lac起源.

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    Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors

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    相关实验视频

    Last Updated: Jul 15, 2026

    Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
    09:02

    Subcloning Plus Insertion (SPI) - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

    Published on: January 8, 2015

    Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells
    12:09

    Transient Expression and Cellular Localization of Recombinant Proteins in Cultured Insect Cells

    Published on: April 20, 2017

    Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors
    06:41

    Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors

    Published on: October 20, 2023

  • 其他序列安排导致了较低,不太了解压缩器生产水平.

    • 结论:

    • 乳酸促进剂可以有效地驱动细菌的lambda抑制剂在大肠杆菌中的合成.
    • 混合核糖体结合部位可以显著增强基因表达.
    • 这种促进者融合策略有可能在大肠杆菌中实现各种基因的高水平表达.
    • 需要进一步的研究来阐明某些结构中影响抑制器生产的因素.