法布二度糖反应抗体是自然抗体的结构类别
Wilton B Williams1, R Ryan Meyerhoff1, R J Edwards1
1Duke Human Vaccine Institute, Durham, NC 27710, USA; Department of Medicine, Duke University, Durham, NC 27710, USA.
Cell
|May 21, 2021
概括
研究人员在中发现了新的糖结合抗体 (Abs),这些抗体针对HIV-1和SARS-CoV-2等多种病原体. 这些天然抗体来自特定的B细胞群体,为免疫反应提供了新的见解.
科学领域:
- 免疫学
- 病毒学
- 葡萄糖生物学
背景情况:
- 自然抗体 (Abs) 可以针对病原体表面的宿主甘氨酸.
- 在识别各种病原体的过程中,
- 广泛中和抗体 (bnAb) 2G12针对HIV-1 Env上的保存的糖贴片.
研究的目的:
- 在 rhesus 和人类中研究甘氨酸反应性 B 细胞的进化.
- 针对HIV-1 Env的新型广泛中和抗体 (bnAbs) 的特征.
- 探索碎片抗原结合 (Fab) -二元化糖 (FDG) -反应性bnAbs的起源和特异性.
主要方法:
- 使用糖化HIV-1包裹 (Env) 作为模型抗原研究了糖素反应性B细胞的进化.
- 描述了感染人免疫缺陷病毒 (SHIV) 的HIV-1EnvFab-二元化甘氨酸 (FDG) 反应性bnAbs.
- 分析了FDG Abs对各种病原体的反应性,包括酵母和SARS-CoV-2尖端蛋白.
主要成果:
- 在2G12中缺少VH交换域的中发现了新的FDG-反应性bnAbs.
- 证明FDG Abs能够识别包括SARS-CoV-2在内的各种病原体中的细胞表面甘氨酸.
- 显示FDG前体在暴露于含有甘氨酸的免疫原体时会扩大,并且在初学者中很丰富.
- 描述FDG前体主要是突变的IgM+IgD+CD27+B细胞.
结论:
- 新型Fab-二元化甘氨酸-反应性广泛中和抗体 (bnAbs) 独立于VH交换域存在.
- 这些天然抗体对包括病毒和酵母在内的各种病原体具有广泛的反应性.
- FDG bnAb前体可能来自抗原经验的IgM+或边缘区域B细胞,在遇到甘氨酸免疫原时会扩大.
相关概念视频
Antibody Structure
62.2K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
62.2K
Antibody Structure and Classes
7.3K
Antibodies, also known as immunoglobulins, are produced by B cells in response to foreign substances, such as bacteria and viruses. These proteins are critical for recognizing and neutralizing these substances, protecting the body from potential harm.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
The basic structure of an antibody consists of four protein chains: two identical heavy chains and two identical light chains. These chains are held together by disulfide bonds and other non-covalent interactions, forming a Y-shaped structure.
7.3K
Immunoglobulin-like Cell Adhesion Molecules
3.7K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.7K


