GSDMB的致病性无化抑制了NK细胞的杀菌功能
Justin M Hansen1, Maarten F de Jong1, Qi Wu1
1Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Cell
|May 22, 2021
概括
通过识别脂,Gasdermin B (GSDMB) 可以直接杀死阴性细菌. 通过降低GSDMB来逃避Shigella flexneri,突出显示了细菌感染免疫中的新型宿主-病原体冲突.
科学领域:
- 免疫学
- 微生物学
- 细胞生物学
背景情况:
- 气体皮质B (GSDMB) 是一个参与炎症细胞死亡的毛孔形成蛋白质.
- GSDMB在宿主防御病原体中的作用尚不清楚.
- GSDMB与人类疾病之间存在遗传联系.
研究的目的:
- 研究GSDMB和Shigella flexneri之间的宿主-病原体相互作用.
- 阐明Shigella逃避GSDMB介导免疫的机制.
- 确定GSDMB的直接抗菌活性.
主要方法:
- 研究了GSDMB和Shigella的IpaH7.8效应蛋白之间的相互作用.
- 研究了GSDMB的无处不在和蛋白质体降解.
- 评估了GSDMB对格拉姆阴性细菌的活性及其在自然杀手细胞反应中的作用.
主要成果:
- 石格拉的IpaH7.8蛋白通过26S蛋白质组对GSDMB进行降解.
- 这种降解抑制了GSDMB的 granzyme A 激活,保护了 Shigella 免受自然杀手细胞的影响.
- 通过向脂,独立于宿主细胞溶解,GSDMB表现出直接的抗菌活性.
结论:
- GSDMB作为一个直接的细胞内抗菌效应剂,向了阴性细菌膜.
- 西格拉采用一种毒性策略来降低GSDMB,避开宿主的免疫反应.
- 这项研究揭示了一种新的病原体逃避机制以及GSDMB在杀死细菌中的直接作用.
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